Evaluation of estrogen receptor Beta binding of pruni cortex and its constituents
| Autor: | Takafumi Fuse, Akira Okonogi, Satoshi Yomoda, Chikafumi Horii, Hiromi Tohno |
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| Rok vydání: | 2010 |
| Předmět: |
Naringenin
China Pharmaceutical Science Genistein Biology Gas Chromatography-Mass Spectrometry Mass Spectrometry Biochanin A Sakuranetin chemistry.chemical_compound Japan Cortex (anatomy) Acne Vulgaris medicine Estrogen Receptor beta Humans Arctigenin Estrogen receptor beta Pharmacology Flavonoids Traditional medicine Plant Extracts medicine.anatomical_structure chemistry Genkwanin Flavanones Prunus Chromatography Liquid Phytotherapy Protein Binding |
| Zdroj: | Yakugaku zasshi : Journal of the Pharmaceutical Society of Japan. 130(7) |
| ISSN: | 0031-6903 |
| Popis: | The bark of Prunus jamasakura Siebold (Pruni Cortex) has long been used in Japan as a folk remedy and is one of ingredients of the Kampo formula, Jumi-haidoku-to (JHT). JHT is used for treatment of skin diseases such as acne (acne vulgaris). According to Kampo medicinal sources, Quercus Cortex can be used in place of Pruni Cortex. In this study, we found that water extracts of Pruni Cortex, not Quercus Cortex showed a binding effect on estrogen receptor beta (ERbeta). Thus, their chemical analysis was carried out by GC-MS and found that five unique constituents (i.e., sakuranetin, naringenin, genistein, genkwanin and arctigenin) were detected in Pruni Cortex. The ERbeta binding capacity of these constituents was examined using 70 ng/ml 17beta-estradiol, as the positive control with 100% ERbeta binding. Among them, genistein (60% at 10 ng/ml) showed the strongest binding capacity than naringenin (60% at 1000 ng/ml) and sakuranetin (40% at 1000 ng/ml). These results suggested that Pruni Cortex in JHT could play an important role in treatment of acne. In addition, those of Pruni Cortex from different harvest places were also examined in their chemical contents and ERbeta binding capacity. The extracts of Pruni Cortex from Kyushu in Japan and Anhui Province in China showed higher contents of genistein and stronger ERbeta binding capacity than that of Pruni Cortex from Tokushima Prefecture in Japan. |
| Databáze: | OpenAIRE |
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