Quantitative analysis of axon collaterals of single pyramidal cells of the anterior piriform cortex of the guinea pig
| Autor: | Daniela Litscher, Masaru Kuroda, Yang Liu, Ingrid Gaischek, Qiang Tang, Satoko Oda, Ting Zhang, Zemin Sheng, Junli Yang, Gerhard Litscher, Wenhai Guo, Masaaki Takayanagi, Kiyoshi Kishi, Irmgard Th. Lippe, Lu Wang, Zhongren Sun |
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| Rok vydání: | 2017 |
| Předmět: |
Male
Photomicrography 0301 basic medicine Olfactory system Axon collaterals Guinea Pigs Piriform Cortex Anterior piriform cortex Biology Guinea pig 03 medical and health sciences Cellular and Molecular Neuroscience chemistry.chemical_compound 0302 clinical medicine Piriform cortex Biocytin medicine Animals Axon Cell Size Lysine Pyramidal Cells General Neuroscience Odor discrimination Olfactory Pathways Olfactory Bulb Single neurons Neural network Axons Olfactory bulb 030104 developmental biology medicine.anatomical_structure nervous system chemistry Olfactory cortex Female Neuroscience 030217 neurology & neurosurgery Research Article |
| Zdroj: | BMC Neuroscience |
| ISSN: | 1471-2202 |
| DOI: | 10.1186/s12868-017-0342-7 |
| Popis: | Background The role of the piriform cortex (PC) in olfactory information processing remains largely unknown. The anterior part of the piriform cortex (APC) has been the focus of cortical-level studies of olfactory coding, and associative processes have attracted considerable attention as an important part in odor discrimination and olfactory information processing. Associational connections of pyramidal cells in the guinea pig APC were studied by direct visualization of axons stained and quantitatively analyzed by intracellular biocytin injection in vivo. Results The observations illustrated that axon collaterals of the individual cells were widely and spatially distributed within the PC, and sometimes also showed a long associational projection to the olfactory bulb (OB). The data showed that long associational axons were both rostrally and caudally directed throughout the PC, and the intrinsic associational fibers of pyramidal cells in the APC are omnidirectional connections in the PC. Within the PC, associational axons typically followed rather linear trajectories and irregular bouton distributions. Quantitative data of the axon collaterals of two pyramidal cells in the APC showed that the average length of axonal collaterals was 101 mm, out of which 79 mm (78% of total length) were distributed in the PC. The average number of boutons was 8926 and 7101, respectively, with 79% of the total number of boutons being distributed in the PC. The percentage of the total area of the APC and the posterior piriform cortex occupied by the average distribution region of the axon collaterals of two superficial pyramidal (SP) cells was about 18 and 5%, respectively. Conclusion Our results demonstrate that omnidirectional connection of pyramidal cells in the APC provides a substrate for recurrent processes. These findings indicate that the axon collaterals of SP cells in the PC could make synaptic contacts with all granule cells in the OB. This study provides the morphological evidence for understanding the mechanisms of information processing and associative memory in the APC. Electronic supplementary material The online version of this article (doi:10.1186/s12868-017-0342-7) contains supplementary material, which is available to authorized users. |
| Databáze: | OpenAIRE |
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