Preparation of hair beads and hair follicle germs for regenerative medicine
| Autor: | Tatsuto Kageyama, Lei Yan, Shoji Maruo, Shimizu Akihiro, Junji Fukuda |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2019 |
| Předmět: |
Biophysics
Mice Nude Bioengineering 02 engineering and technology Dermal papilla cell Regenerative Medicine Regenerative medicine Biomaterials Mesoderm 03 medical and health sciences Spheroids Cellular medicine Animals Humans 030304 developmental biology Cell Aggregation Hair follicle germ 0303 health sciences Attraction force Microgels integumentary system Chemistry Mesenchymal stem cell Epithelial Cells Dermis 021001 nanoscience & nanotechnology Hair follicle medicine.disease Embryonic stem cell Cell biology Transplantation Mice Inbred C57BL medicine.anatomical_structure Dermal papillae Hair loss Collagen microgel Gene Expression Regulation Hair regenerative medicine Mechanics of Materials Ceramics and Composites Collagen Stem cell 0210 nano-technology Hair Follicle Biomarkers |
| Zdroj: | Biomaterials. 212:55-63 |
| ISSN: | 0142-9612 |
| Popis: | Hair regenerative medicine is a promising approach for hair loss, during which autologous follicular stem cells are transplanted into regions of hair loss to regenerate hairs. Because cells transplanted as a single cell suspension scarcely generate hairs, the engineering of three-dimensional (3D) tissues before transplantation has been explored to improve this process. Here, we propose an approach to fabricate collagen-enriched cell aggregates, named hair beads (HBs), through the spontaneous constriction of cell-encapsulated collagen drops. Mouse embryonic mesenchymal cells or human dermal papilla cells were encapsulated in 2-μl collagen microgels, which were concentrated >10-fold in volume during 3 days of culture. Interestingly, HB constriction was attributed to attraction forces driven by myosin II and involved the upregulation of follicular genes. Single HBs with epithelial cells seeded in U-shaped microwells formed dumbbell-like structures comprising respective aggregates (named bead-based hair follicle germs, bbHFGs), during 3 days of culture. bbHFGs efficiently generated hair follicles upon intracutaneous transplantation into the backs of nude mice. Using an automated spotter, this approach was scalable to prepare a large number of bbHFGs, which is important for clinical applications. Therefore, this could represent a robust and practical approach for the preparation of germ-like tissues for hair regenerative medicine. |
| Databáze: | OpenAIRE |
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