Clinical Importance of Placental Testing among Suspected Cases of Congenital Zika Syndrome

Autor: Kjersti Aagaard, Catherine Eppes, James Versalovic, Michael A. Belfort, Magdalena Sanz-Cortes, Brianna Sanchez, Gregory Valentine, Martha Rac, Angela Major, Gail J. Demmler-Harrison, Kenneth L. Muldrew, Michelle Turley, Eumenia Costa da Cunha Castro, Tiffany F. Kautz, James J Dunn, Timothy Stout, Maxim Seferovic
Jazyk: angličtina
Rok vydání: 2019
Předmět:
0301 basic medicine
Microcephaly
congenital Zika syndrome
placental Zika infection
Placenta
Umbilical cord
Serology
Zika virus
lcsh:Chemistry
0302 clinical medicine
Pregnancy
placental testing
030212 general & internal medicine
microcephaly
Pregnancy Complications
Infectious
lcsh:QH301-705.5
Spectroscopy
biology
Zika Virus Infection
Brain
Syndrome
General Medicine
Immunohistochemistry
Magnetic Resonance Imaging
3. Good health
Computer Science Applications
Phenotype
Real-time polymerase chain reaction
medicine.anatomical_structure
molecular virology
Female
Symptom Assessment
viral pathogenicity
Adult
Arbovirus
Ultrasonography
Prenatal
Article
Catalysis
congenital brain malformations
Inorganic Chemistry
Young Adult
03 medical and health sciences
Flaviviridae
medicine
Humans
Physical and Theoretical Chemistry
Molecular Biology
business.industry
Organic Chemistry
medicine.disease
biology.organism_classification
Virology
Infectious Disease Transmission
Vertical
030104 developmental biology
arbovirus
lcsh:Biology (General)
lcsh:QD1-999
business
Zdroj: International Journal of Molecular Sciences, Vol 20, Iss 3, p 712 (2019)
International Journal of Molecular Sciences
Volume 20
Issue 3
ISSN: 1422-0067
Popis: Contemporaneous Zika virus (ZIKV) strains can cause congenital Zika syndrome (CZS). Current ZIKV clinical laboratory testing strategies are limited and include IgM serology (which may wane 12 weeks after initial exposure) and nucleic acid testing (NAT) of maternal serum, urine, and placenta for (+) strand ZIKV RNA (which is often transient). The objectives of this study were to determine if use of additional molecular tools, such as quantitative PCR and microscopy, would add to the diagnostic value of current standard placental ZIKV testing in cases with maternal endemic exposure and indeterminate testing. ZIKV RNA was quantified from dissected sections of placental villi, chorioamnion sections, and full cross-sections of umbilical cord in all cases examined. Quantitation with high-resolution automated electrophoresis determined relative amounts of precisely verified ZIKV (74-nt amplicons). In order to localize and visualize stable and actively replicating placental ZIKV in situ, labeling of flaviviridae glycoprotein, RNA ISH against both (+) and (&ndash
) ZIKV-specific ssRNA strands, and independent histologic examination for significant pathologic changes were employed. We demonstrate that the use of these molecular tools added to the diagnostic value of placental ZIKV testing among suspected cases of congenital Zika syndrome with poorly ascribed maternal endemic exposure.
Databáze: OpenAIRE
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