Suggestive evidence of genetic association of −572G > C polymorphism with primary open angle glaucoma in a North Indian Punjabi population
| Autor: | Sanjana Mehrotra, Archna Pruthi, Rashim Mannan, Nanamika Thakur, Vipin Kumar, Rajeev Kumar Pandey |
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| Rok vydání: | 2021 |
| Předmět: |
Adult
Male medicine.medical_specialty Linkage disequilibrium Genotype genetic structures Haploview Immunology Population India Enzyme-Linked Immunosorbent Assay Polymorphism Single Nucleotide Linkage Disequilibrium Gene Frequency Internal medicine Genetic model medicine Genetic predisposition Humans Immunology and Allergy Genetic Predisposition to Disease education Alleles Aged Genetic association education.field_of_study Interleukin-6 business.industry Sequence Analysis DNA General Medicine Middle Aged eye diseases Genotype frequency Case-Control Studies Population Surveillance Female business Glaucoma Open-Angle |
| Zdroj: | Human Immunology. 82:791-797 |
| ISSN: | 0198-8859 |
| DOI: | 10.1016/j.humimm.2021.06.009 |
| Popis: | Background IL6 is an important candidate gene implicated in the pathogenesis of glaucoma. The present study assessed the genetic association of −174G > C and −572G > C polymorphisms in the IL6 promoter region with primary open angle glaucoma (POAG) and primary angle closure glaucoma (PACG) in a north Indian Punjabi cohort. Methods 910 subjects (313 POAG, 148 PACG cases and 449 controls) were recruited. Genotyping was done by TaqMan assays. Genetic association was tested under different genetic models using Plink. Diplotype and linkage disequilibrium (LD) analysis was done through Haploview. Association of clinical parameters with the genotypes was assessed by one-way ANOVA. Adjustment for potential confounding variables was done by binary logistic regression. IL6 levels were measured in POAG patients and controls. Results 572G > C variant showed marginal difference in genotype frequency between pooled cases and POAG subgroup with respect to controls (p = 0.042; OR = 1.33; and p = 0.041; OR = 1.37). The GC genotype conferred 1.37-fold protection under codominant model in POAG cases (p = 0.034, OR = 1.37, 95% CI = 1.02–1.85; pcorr = 0.025, OR = 1.45, 95% CI = 1.04–2.02). The mean value for IOP was elevated among cases having ‘CC’ genotype at the −572G > C locus (p = 0.037). Lower levels of IL6 were detected in POAG patients in plasma samples (p = 0.0001). Conclusion The study reports suggestive evidence for −572G > C variant in IL6 in affecting genetic susceptibility to POAG in the targeted North Indian Punjabi cohort. A correlation of IL6 levels in aqueous humor (AH) and systemic circulation in POAG was observed, the functional and diagnostic relevance of which may be further investigated. |
| Databáze: | OpenAIRE |
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