Enzyme-linked immunosorbent assay-enzyme-linked coagulation assay for detection of antibodies to Clostridium botulinum neurotoxins A, B, and E and solution-phase complexes
| Autor: | George J. Doellgast, G A Beard, M X Triscott, J D Bottoms |
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| Jazyk: | angličtina |
| Rok vydání: | 1994 |
| Předmět: |
Microbiology (medical)
Botulinum Toxins Enzyme-Linked Immunosorbent Assay medicine.disease_cause Binding Competitive Sensitivity and Specificity Mice Antibody Specificity Neutralization Tests medicine Clostridium botulinum Neurotoxin Animals Humans Clostridiaceae Horses chemistry.chemical_classification biology Toxin biology.organism_classification Molecular biology Antibodies Bacterial Titer Enzyme chemistry Polyclonal antibodies Evaluation Studies as Topic biology.protein Biological Assay Antibody Chickens Research Article |
| Popis: | The solution-phase complex assay for toxins A, B, and E from Clostridium botulinum (Elcatech, Inc., Winston-Salem, N.C.) was modified to measure antibody. The addition of unlabeled polyclonal antibodies to a mixture consisting of toxin with chicken antibody and RVV-XA-labeled horse antibody reduces the sensitivity of detection of neurotoxin. This reduction in sensitivity can be used as a measure of the specific antibody titer. |
| Databáze: | OpenAIRE |
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