Evaluation of an immunoisolation membrane formed by incorporating a polyvinyl alcohol hydrogel within a microporous filter support
| Autor: | Ronald L. Fournier, Andrew R. Baker, Jennifer L. Long, James M. Horner, Peter J. Goldblatt, Steven H. Selman, Jeffrey G. Sarver |
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| Rok vydání: | 1997 |
| Předmět: |
Male
0301 basic medicine Materials science Membrane permeability Cell Transplantation Biomedical Engineering lcsh:Medicine Biocompatible Materials macromolecular substances Polyvinyl alcohol Permeability Rats Sprague-Dawley 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Animals Molecule Porosity Peritoneal Cavity Transplantation Micropore Filters lcsh:R Temperature technology industry and agriculture Sterilization Membranes Artificial Cell Biology Microporous material Rats 030104 developmental biology Membrane Chemical engineering chemistry Polyvinyl Alcohol Glutaraldehyde Selectivity Gels 030217 neurology & neurosurgery |
| Zdroj: | Cell Transplantation, Vol 6 (1997) |
| ISSN: | 0963-6897 |
| DOI: | 10.1016/s0963-6897(97)00119-x |
| Popis: | An immunoisolation membrane formed by incorporating a high water content polyvinyl alcohol (PVA) hydrogel into a microporous polyether sulfone (PES) filter has been investigated in this study. The PVA hydrogel is formed in situ within the filter pores via glutaraldehyde (GA) crosslinking under acidic conditions. The tortuous nature of the microporous filter pores securely anchors the embedded hydrogel to provide excellent structural integrity. The high void fraction of the PES filter support (>80%) and high water content of the PVA hydrogel (>85% water by weight) allow excellent solute transport rates, while an appropriate level of glutaraldehyde crosslinking supplies the required molecular size selectivity. In vitro permeability measurements made with solutes covering a wide range of molecular sizes demonstrate high transport rates for small nutrient molecules with rapidly diminishing permeabilities above a molecular weight of approximately 1,000 Dalton. Implantation experiments show that the membrane properties are not deleteriously affected by prolonged in vivo exposure or common sterilization techniques. Thus, this hybrid hydrogel/filter membrane system offers a promising approach to the immunoisolation of implanted cells. |
| Databáze: | OpenAIRE |
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