Angiogenesis in pterygium: study of microvessel density, vascular endothelial growth factor, and thrombospondin-1
| Autor: | Vassiliki Malamou-Mitsi, Spiridon Gorezis, Elli Ioachim, Miltiadis Aspiotis, E. Tsanou, Angelos Skyrlas, Maria Stefaniotou |
|---|---|
| Rok vydání: | 2006 |
| Předmět: |
Male
Pathology medicine.medical_specialty Angiogenesis Pterygium Microcirculation Thrombospondin 1 Neovascularization chemistry.chemical_compound medicine Humans Vascular Endothelial Growth Factors/*analysis Thrombospondin 1/*metabolism Aged Retrospective Studies Microvessel density Aged 80 and over Thrombospondin Microcirculation/pathology Neovascularization Pathologic Vascular Endothelial Growth Factors business.industry Neovascularization Pathologic/*pathology Conjunctiva/blood supply/*chemistry Middle Aged medicine.disease eye diseases Vascular endothelial growth factor Ophthalmology Pterygium/*etiology/metabolism/pathology chemistry cardiovascular system Female sense organs medicine.symptom business Conjunctiva |
| Zdroj: | Eye. 21:1095-1101 |
| ISSN: | 1476-5454 0950-222X |
| DOI: | 10.1038/sj.eye.6702495 |
| Popis: | PURPOSE: This retrospective study aims to elucidate the role of angiogenesis in the pathogenesis of pterygium. We evaluated microvessel density (MVD), and expression of vascular endothelial growth factor (VEGF) and thrombospondin-1 (TSP-1). METHODS: Fifty-two surgically excised pterygia and seven normal conjunctivae were immunohistochemically studied applying the streptavidin-biotin method in paraffin-embedded tissue sections. Monoclonal antibodies were targeted against CD31, VEGF, and TSP-1 proteins. Results: Pterygium presented with statistically significant higher average count of microvessels compared to normal conjunctivae (17.97+/-8.5 vs5.72+/-5 per high power field, P=0.001). In 24/52 (46.2%) cases of pterygium, high expression levels for VEGF were demonstrated, whereas the mean percentage of VEGF-positive epithelial cells was 58.03%. Furthermore, normal conjunctival presented statistically significant higher expression levels for VEGF in epithelial cells (83.14+/-36.08 vs58.03+/-31.23%, P=0.007). On the contrary, the presence of VEGF immunoreactivity in vascular endothelial and stromal cells was significantly higher in pterygium tissues (P |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|