Exploring the fundamental role of potassium channels in novel model plants
| Autor: | Ariela Vergara-Jaque, Janin Riedelsberger, Wendy González, Ingo Dreyer |
|---|---|
| Rok vydání: | 2019 |
| Předmět: |
0106 biological sciences
0301 basic medicine root to shoot translocation Potassium Channels Physiology Protein Conformation Potassium new physiological properties chemistry.chemical_element Plant Science Flowers 01 natural sciences phloem root lateral primordium 03 medical and health sciences new tissue location sites Plants Research Papers Potassium channel potassium Shaker channel 030104 developmental biology chemistry Potassium Channels Voltage-Gated Biophysics Growth and Development 010606 plant biology & botany |
| Zdroj: | Journal of Experimental Botany |
| ISSN: | 1460-2431 |
| Popis: | The unexpected location of VvK5.1 expression detected in the lateral root primordium, berry phloem and pistil provides new insights into the roles that this outward channel type can play in plants. Grapevine (Vitis vinifera L.), one of the most important fruit crops, is a model plant for studying the physiology of fleshy fruits. Here, we report on the characterization of a new grapevine Shaker-type K+ channel, VvK5.1. Phylogenetic analysis revealed that VvK5.1 belongs to the SKOR-like subfamily. Our functional characterization of VvK5.1 in Xenopus oocytes confirms that it is an outwardly rectifying K+ channel that displays strict K+ selectivity. Gene expression level analyses by real-time quantitative PCR showed that VvK5.1 expression was detected in berries, roots, and flowers. In contrast to its Arabidopsis thaliana counterpart that is involved in K+ secretion in the root pericycle, allowing root to shoot K+ translocation, VvK5.1 expression territory is greatly enlarged. Using in situ hybridization we showed that VvK5.1 is expressed in the phloem and perivascular cells of berries and in flower pistil. In the root, in addition to being expressed in the root pericycle like AtSKOR, a strong expression of VvK5.1 is detected in small cells facing the xylem that are involved in lateral root formation. This fine and selective expression pattern of VvK5.1 at the early stage of lateral root primordia supports a role for outward channels to switch on cell division initiation. |
| Databáze: | OpenAIRE |
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