Detection of enteric parasites and molecular characterization of Giardia duodenalis and Blastocystis sp. in patients admitted to hospital in Ankara, Turkey
| Autor: | Funda Dogruman-Al, Alejandro Dashti, David Carmena, Filiz Demirel-Kaya, Begoña Bailo, Pamela C. Köster, Fakhriddin Sarzhanov |
|---|---|
| Přispěvatelé: | Gazi University, Instituto de Salud Carlos III |
| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
Turkey 030106 microbiology Dientamoeba fragilis Cryptosporidium Microbiology 03 medical and health sciences Entamoeba histolytica Helminths Intestinal parasites Blastocystis biology Molecular epidemiology Multilocus genotyping biology.organism_classification 030104 developmental biology Infectious Diseases Giardia duodenalis Blastocystis sp Animal Science and Zoology Parasitology |
| Zdroj: | Parasitology. 148:550-561 |
| ISSN: | 1469-8161 0031-1820 |
| Popis: | This epidemiological study assesses the occurrence of enteric parasites in 4303 patients attended at two public hospitals in Ankara (Turkey) during 2018–2019. Microscopy was used as a screening test. Giardia duodenalis was also identified using a commercial ELISA for the detection of parasite-specific coproantigens. Giardia-positive samples by microscopy/ELISA were confirmed by real-time PCR and characterized using a multilocus genotyping scheme. Blastocystis sp. was genotyped in a sample subset. Blastocystis sp. (11.1%, 95% CI 11.4‒14.8%) and G. duodenalis (1.56%, 95% CI 1.22‒1.96) were the most prevalent pathogens found. Cryptosporidium spp., Entamoeba histolytica and intestinal helminths were only sporadically (G. duodenalis, sequence (n = 30) analyses revealed the presence of sub-assemblages AII (23.3%), discordant AII/AIII (23.3%) and mixed AII + AIII (6.7%) within assemblage A, and BIII (10.0%), BIV (3.3%) and discordant BIII/BIV (23.3%) within assemblage B. Two additional sequences (6.7%) were assigned to the latter assemblage but sub-assemblage information was unknown. No associations between G. duodenalis assemblages/sub-assemblages and sociodemographic and clinical variables could be demonstrated. For Blastocystis sp., sequence (n = 6) analyses identified subtypes ST1, ST2 and ST3 at equal proportions. This is the first molecular characterization of G. duodenalis based on MLG conducted in Turkey to date. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|