Insulin signaling acts in adult adipocytes via GSK-3β and independently of FOXO to control Drosophila female germline stem cell numbers
| Autor: | Alissa R. Armstrong, Daniela Drummond-Barbosa |
|---|---|
| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Male medicine.medical_treatment Cell Count Nutrient sensing Germline Article 03 medical and health sciences chemistry.chemical_compound Oogenesis Adipocyte medicine Adipocytes Animals Drosophila Proteins Insulin Molecular Biology Cell Proliferation Glycogen Synthase Kinase 3 beta biology Adult Germline Stem Cells Stem Cells Ovary Forkhead Transcription Factors Cell Biology biology.organism_classification Cell biology Insulin receptor 030104 developmental biology Germ Cells chemistry embryonic structures biology.protein Drosophila Female Drosophila melanogaster Stem cell Proto-Oncogene Proteins c-akt Developmental Biology Adult stem cell Signal Transduction |
| Zdroj: | Developmental biology. 440(1) |
| ISSN: | 1095-564X |
| Popis: | Tissue-specific stem cells are tied to the nutritional and physiological environment of adult organisms. Adipocytes have key endocrine and nutrient-sensing roles and have emerged as major players in relaying dietary information to regulate other organs. For example, previous studies in Drosophila melanogaster revealed that amino acid sensing as well as diet-dependent metabolic pathways function in adipocytes to influence the maintenance of female germline stem cells (GSCs). How nutrient-sensing pathways acting within adipocytes influence adult stem cell lineages, however, is just beginning to be elucidated. Here, we report that insulin/insulin-like growth factor signaling in adipocytes promotes GSC maintenance, early germline cyst survival, and vitellogenesis. Further, adipocytes use distinct mechanisms downstream of insulin receptor activation to control these aspects of oogenesis, all of which are independent of FOXO. We find that GSC maintenance is modulated by Akt1 through GSK-3β, early germline cyst survival is downstream of adipocyte Akt1 but independent of GSK-3β, and vitellogenesis is regulated through an Akt1-independent pathway in adipocytes. These results indicate that, in addition to employing different types of nutrient sensing, adipocytes can use distinct axes of a single nutrient-sensing pathway to regulate multiple stages of the GSC lineage in the ovary. |
| Databáze: | OpenAIRE |
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