Simultaneous measurement of metabolic activity and membrane integrity in marine bacterioplankton determined by confocal laser-scanning microscopy
| Autor: | Heidemarie Pirker, Clemens Pausz, Karen E. Stoderegger, Gerhard J. Herndl |
|---|---|
| Rok vydání: | 2005 |
| Předmět: |
Cell
Aquatic Science Biology biology.organism_classification law.invention chemistry.chemical_compound medicine.anatomical_structure chemistry Single-cell analysis Biochemistry Confocal microscopy law SYBR Green I Nucleic acid medicine Propidium iodide Leucine Ecology Evolution Behavior and Systematics Bacteria |
| Zdroj: | Scopus-Elsevier |
| ISSN: | 1616-1564 0948-3055 |
| Popis: | We simultaneously assessed the metabolic activity and viability of individual bacterio- plankton cells in the coastal and open North Sea. Three different techniques were applied to deter- mine cell features related to the physiological status of the cell. SYBR Green I was used to estimate the nucleic acid content of the cell. Propidium iodide (PI) stains cells with a compromised cell mem- brane, commonly interpreted as indicative of dead cells. Microautoradiography (MA) with radiola- beled glucose and leucine was applied to indicate metabolically active cells. The relative abundance of metabolically active cells determined by MA was usually |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|