Hypoxia inducible factor 1α down regulates cell surface expression of α1,2-fucosylated glycans in human pancreatic adenocarcinoma cells
| Autor: | Boris Tefsen, Andreas Maus, Sandra J. van Vliet, Tessa D. Nauta, Irma van Die, Ana I. Belo, Pieter Koolwijk, Lisa C. Laan |
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| Přispěvatelé: | Molecular cell biology and Immunology, Physiology, CCA - Immuno-pathogenesis, ICaR - Ischemia and repair |
| Jazyk: | angličtina |
| Předmět: |
Glycan
Glycosylation Cell Biophysics Down-Regulation Adenocarcinoma Biochemistry Downregulation and upregulation Structural Biology Polysaccharides Fucosyltransferase Pancreatic cancer Cell Line Tumor Genetics medicine Humans Regulation of gene expression Hypoxia inducible factor 1 α (HIF-1α) RNA Messenger Molecular Biology Fucose Cancer biology Cell Biology medicine.disease Fucosyltransferases Hypoxia-Inducible Factor 1 alpha Subunit Molecular biology Cell biology Gene Expression Regulation Neoplastic Pancreatic Neoplasms medicine.anatomical_structure Hypoxia-inducible factors biology.protein Glycosyltransferase |
| Zdroj: | FEBS Letters, 589(18), 2359-2366. Wiley-Blackwell Da Silva Aresta Belo, A I, van Vliet, S J, Maus, A, Laan, L C, Nauta, T, Koolwijk, P, Tefsen, B & van Die, I 2015, ' Hypoxia inducible factor 1 alpha down regulates cell surface expression of alpha 1,2-fucosylated glycans in human pancreatic adenocarcinoma cells ', FEBS Letters, vol. 589, no. 18, pp. 2359-2366 . https://doi.org/10.1016/j.febslet.2015.07.035 |
| ISSN: | 0014-5793 |
| DOI: | 10.1016/j.febslet.2015.07.035 |
| Popis: | The α1,2-fucosyltransferase activity in pancreatic tumors is much lower compared to normal pancreatic tissue. Here we show that hypoxia inducible factor (HIF) 1α is constitutively expressed in the pancreatic cancer cell lines Pa-Tu-8988S and Pa-Tu-8988T and suppresses the expression of the α1,2-fucosyltransferase genes FUT1 and FUT2. Down regulation of HIF-1α expression resulted in elevated FUT1 and FUT2 transcript levels and an increased expression of α1,2-fucosylated glycan structures on the surface of these cells. In conclusion, our data are the first to identify HIF-1α as a suppressor of FUT1/2 expression, thereby regulating α1,2-fucosylation of cell-surface glycans. |
| Databáze: | OpenAIRE |
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