Non-specific binding of normal human IgG, including F(ab′)2 and Fc fragments, to embryonic rat brain neurons and human cortex synaptosomes
| Autor: | B. Cassin, David A. Loeffler, Peter A. LeWitt, Gregory Kapatos, C. M. Brickman, M.A. KuKuruga, Michael J. Bannon |
|---|---|
| Rok vydání: | 1992 |
| Předmět: |
Time Factors
Immunology Central nervous system Flow cytometry Immunoglobulin Fab Fragments Fetus Cortex (anatomy) medicine Animals Humans Immunology and Allergy Cerebral Cortex Neurons Synaptosome medicine.diagnostic_test biology Brain Molecular biology Immunoglobulin Fc Fragments Rats medicine.anatomical_structure Neurology IgG binding Cerebral cortex Immunoglobulin G biology.protein Neurology (clinical) Neuron Antibody Synaptosomes |
| Zdroj: | Journal of Neuroimmunology. 38:45-52 |
| ISSN: | 0165-5728 |
| DOI: | 10.1016/0165-5728(92)90089-4 |
| Popis: | Binding of normal human IgG to embryonic rat brain neurons was quantitated by flow cytometry. IgG binding was linear between 0.05 and 1.5 mg/ml; slight binding was detectable even at normal cerebrospinal fluid concentrations. Similar binding curves were obtained for purified Fc and F(ab′) 2 fragments from normal human IgG. Normal human IgG also bound to synaptosomes (resealed nerve terminals) from human cerebral cortex. However, competition assays utilizing 125 I-IgG showed no evidence for specific binding. This study indicates that the specificity of putative anti-neuronal antibodies should be confirmed by competition assays as for other receptor-ligand binding. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|