Identification of conserved genes triggering puberty in European sea bass males (Dicentrarchus labrax) by microarray expression profiling
| Autor: | Berta Crespo, Ana M. Gómez, Mercedes Blázquez, Paula Medina, Silvia Zanuy |
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| Přispěvatelé: | Ministerio de Ciencia e Innovación (España), Universidad de Antofagasta |
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Cell division lcsh:QH426-470 media_common.quotation_subject lcsh:Biotechnology Biology Cell cycle Teleosts Biological pathway Transcriptome 03 medical and health sciences Meiosis lcsh:TP248.13-248.65 Genetics Retinoic acid Sea bass Spermatogenesis media_common Cell biology lcsh:Genetics 030104 developmental biology Reproduction Research Article Biotechnology |
| Zdroj: | BMC Genomics, Vol 18, Iss 1, Pp 1-17 (2017) BMC Genomics Digital.CSIC. Repositorio Institucional del CSIC instname |
| Popis: | 17 pages, 7 figures, 2 tables, additional files.-- The complete design was deposited at the Gene Expression Omnibus NCBI database GSE47400 https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?token=exgnmkgcfbwnhkp&acc=GSE47400 and the platform that validates the microarray can be found at GPL13443.-- Erratum to: Identification of conserved genes triggering puberty in European sea bass males (Dicentrarchus labrax) by microarray expression profiling, BMC Genomics 18(1): 513 (2017), https://doi.org/10.1186/s12864-017-3909-x Spermatogenesis is a complex process characterized by the activation and/or repression of a number of genes in a spatio-temporal manner. Pubertal development in males starts with the onset of the first spermatogenesis and implies the division of primary spermatogonia and their subsequent entry into meiosis. This study is aimed at the characterization of genes involved in the onset of puberty in European sea bass, and constitutes the first transcriptomic approach focused on meiosis in this species. [Results] European sea bass testes collected at the onset of puberty (first successful reproduction) were grouped in stage I (resting stage), and stage II (proliferative stage). Transition from stage I to stage II was marked by an increase of 11ketotestosterone (11KT), the main fish androgen, whereas the transcriptomic study resulted in 315 genes differentially expressed between the two stages. The onset of puberty induced 1) an up-regulation of genes involved in cell proliferation, cell cycle and meiosis progression, 2) changes in genes related with reproduction and growth, and 3) a down-regulation of genes included in the retinoic acid (RA) signalling pathway. The analysis of GO-terms and biological pathways showed that cell cycle, cell division, cellular metabolic processes, and reproduction were affected, consistent with the early events that occur during the onset of puberty. Furthermore, changes in the expression of three RA nuclear receptors point at the importance of the RA-signalling pathway during this period, in agreement with its role in meiosis. [Conclusion] The results contribute to boost our knowledge of the early molecular and endocrine events that trigger pubertal development and the onset of spermatogenesis in fish. These include an increase in 11KT plasma levels and changes in the expression of several genes involved in cell proliferation, cell cycle progression, meiosis or RA-signalling pathway. Moreover, the results can be applied to study meiosis in this economically important fish species for Mediterranean countries, and may help to develop tools for its sustainable aquaculture. This work was supported by Aquagenomics project (CSD2007-0002) from the Spanish Ministry of Science and Innovation to SZ and a MICINN project (AGL2011-28890) to AG. PM was supported by a grant from the University of Antofagasta MECE2 (ANT0806) |
| Databáze: | OpenAIRE |
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