Paraoxonase 1 and platelet-activating factor acetylhydrolase activities in patients with low hdl-cholesterol levels with or without primary hypertriglyceridemia
| Autor: | Graciela Castro, Julián Verona, R Wikinski, Laura Schreier, Jean-Charles Fruchart, Fernando Brites |
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| Rok vydání: | 2003 |
| Předmět: |
Adult
Male medicine.medical_specialty Time Factors Apolipoprotein B Lipoproteins Antioxidants chemistry.chemical_compound High-density lipoprotein Internal medicine medicine Humans Hypoalphalipoproteinemia Aged Hypertriglyceridemia biology Cholesterol business.industry Aryldialkylphosphatase Cholesterol HDL Paraoxonase nutritional and metabolic diseases General Medicine Cholesterol LDL Middle Aged medicine.disease Lipid Metabolism PON1 Endocrinology Phenotype chemistry 1-Alkyl-2-acetylglycerophosphocholine Esterase biology.protein lipids (amino acids peptides and proteins) business Lipoprotein |
| Zdroj: | Archives of medical research. 35(3) |
| ISSN: | 0188-4409 |
| Popis: | Background Previous studies have shown that high density lipoprotein (HDL)-deficient states are associated with reduced paraoxonase 1 (PON1) activity. However, HDL reduction caused by primary hypertriglyceridemia has not been fully explored. The aim of the present study was to evaluate whether PON1 and platelet-activating factor acetylhydrolase (PAF-AH), two antioxidant enzymes, were altered in patients with low HDL-cholesterol levels with or without primary hypertriglyceridemia in comparison with control normolipemic subjects. Methods We studied 24 patients with low HDL-cholesterol levels with ( n = 12) or without ( n = 12) primary hypertriglyceridemia in comparison with 12 control subjects who presented normal HDL-cholesterol and triglyceride levels. Paraoxon and phenylacetate were used as substrate for measuring PON1 activities and 1-hexadecyl-2-[ 3 H]acetyl-glycero-3-phosphocholine for platelet-activating factor acetylhydrolase (PAF-AH) activity. Double substrate method was used to assign phenotypes. Lipid, lipoprotein, apolipoprotein, and lipoprotein particles were determined by standardized methods. Results Both PON1 activities were significantly reduced in patients with low HDL-cholesterol levels. This reduction could be selectively attributed to the hypertriglyceridemic subgroup. PAF-AH activity was not different between hypoalphalipoproteinemic patients and controls. PON1 activities correlated positively and significantly with HDL-cholesterol, HDL 2 -cholesterol, HDL 3 -cholesterol, HDL-phospholipids, apo A-I, apo A-II, and LpA-I:A-II. PAF-AH correlated positively and significantly with total and low density lipoprotein-cholesterol. Conclusions Data from this study would suggest that in hypoalphalipoproteinemic syndrome, particularly when associated with hypertriglyceridemia, there is impairment in enzymatic antioxidant activity exclusively related with HDL. |
| Databáze: | OpenAIRE |
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