Role of 5′-Guanylylimidodiphosphate in the Activation of Adenylyl Cyclase by Parathyroid Hormone
| Autor: | John T. Potts, Geoffrey William Tregear, Edward N. Callahan, David Goltzman |
|---|---|
| Rok vydání: | 1976 |
| Předmět: |
medicine.medical_specialty
Kidney Cortex Membranes GTP' ADCY9 Antagonist Parathyroid hormone Stimulation General Medicine Cyclase ADCY10 Enzyme Activation Adenylyl cyclase chemistry.chemical_compound Dogs Endocrinology chemistry Parathyroid Hormone Internal medicine Cyclic AMP medicine Animals Cattle Guanosine Triphosphate Adenylyl Cyclases |
| Zdroj: | Endocrine Research Communications. 3:407-419 |
| ISSN: | 0093-6391 |
| DOI: | 10.3109/07435807609073913 |
| Popis: | We have studied the effects of guanylylimidodiphosphate (Gpp(NH)p), an analogue of GTP, on the stimulation of renal cortical adenylyl cyclase by bovine parathyroid hormone (bPTH, or bPTH-(1-84)). Incubation of canine renal membranes with bPTH-(3-34), a specific antagonist of parathyroid hormone, in either the presence or absence of Gpp(NH)p, prevented subsequently added bPTH-(1-84) from stimulating adenylyl cyclase. The addition of the antagonist to a cyclase system previously activated by both bPTH-(1-84) and Gpp(NH)p, however, produced no inhibition of enzyme activity. Removal of bPTH by washing the membranes virtually abolished activity, but washing after addition of bPTH plus Gpp(NH)p did not prevent continued accumulation of cAMP. The persistence of the activity of the enzyme brought about by the addition of Gpp(NH)p plus bPTH, despite washing or addition of specific inhibitor of bPTH action, indicates that the activity of the hormone-specific adenylyl cyclase in membrane suspensions is independent of cintinuous occupancy of the peptide-hormone receptor by bPTH in the presence of the guanyl-nucleotide analogue. |
| Databáze: | OpenAIRE |
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