Sinoacutine inhibits inflammatory responses to attenuates acute lung injury by regulating NF-κB and JNK signaling pathways
| Autor: | Yun-Kuan Liu, Xing Xin Yang, Liyuan Zhu, Chaorui Peng, Zixian Yang, Yun-Shu Ma, Lili Cui, Dan-Ye Li, Cai Junfei, Yuancui Zhao, Sun Xingqian |
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| Rok vydání: | 2021 |
| Předmět: |
MAPK/ERK pathway
Male China MAP Kinase Kinase 4 MAP Kinase Signaling System p38 mitogen-activated protein kinases Acute Lung Injury Anti-Inflammatory Agents Lung injury Pharmacology NF-κB Nitric oxide chemistry.chemical_compound Other systems of medicine Mice Animals Outbred Strains Animals biology Molecular Structure Kinase Plant Extracts Research Sinoacutine NF-kappa B MAPK Nitric oxide synthase RAW 264.7 Cells Complementary and alternative medicine chemistry Morphinans biology.protein Signal transduction Anti-inflammatory RZ201-999 RAW264.7 macrophages |
| Zdroj: | BMC Complementary Medicine and Therapies BMC Complementary Medicine and Therapies, Vol 21, Iss 1, Pp 1-10 (2021) |
| ISSN: | 2662-7671 |
| Popis: | Background Stephania yunnanensis H. S. Lo is widely used as an antipyretic, analgesic and anti-inflammatory herbal medicine in SouthWest China. In this study, we investigated the anti-inflammatory activity and mechanism of sinoacutine (sino), one of the primary components extracted from this plant. Methods A RAW264.7 cell model was established using lipopolysaccharide (LPS) induced for estimation of cytokines in vitro, qPCR was used to estimate gene expression, western blot analysis was used to estimate protein level and investigate the regulation of NF- κB, JNK and MAPK signal pathway. In addition, an acute lung injury model was established to determine lung index and levels of influencing factors. Results Using the RAW264.7 model, we found that sino reduced levels of nitric oxide (NO), tumour necrosis factor-α (TNF-α), interleukin (IL)-1β and prostaglandin E2 (PGE2) but increased levels of IL-6. qPCR analysis revealed that sino (50, 25 μg/ml) inhibited gene expression of nitric oxide synthase (iNOS). western blot analysis showed that sino significantly inhibited protein levels of both iNOS and COX-2. Further signalling pathway analysis validated that sino also inhibited phosphorylation of p65 in the NF-κB and c-Jun NH2 terminal kinase (JNK) signalling pathways but promoted the phosphorylation of extracellular signal regulated kinase (ERK) and p38 in the MAPK signalling pathway. In addition, in a mouse model induced by LPS, we determined that sino reduced the lung index and the levels of myeloperoxidase (MPO), NO, IL-6 and TNF-α in lung tissues and bronchoalveolar lavage fluid (BALF) in acute lung injury (ALI). Conclusion Taken together, our results demonstrate that sino is a promising drug to alleviate LPS-induced inflammatory reactions. |
| Databáze: | OpenAIRE |
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