Additional file 1 of Comparing syngeneic and autochthonous models of breast cancer to identify tumor immune components that correlate with response to immunotherapy in breast cancer

Autor: Lal, Jessica Castrillon, Townsend, Madeline G., Mehta, Anita K., Oliwa, Madisson, Miller, Eric, Sotayo, Alaba, Cheney, Emily, Mittendorf, Elizabeth A., Letai, Anthony, Guerriero, Jennifer L.
Rok vydání: 2021
DOI: 10.6084/m9.figshare.15122313.v1
Popis: Additional file 1: Supplemental Figure 1. Immunophenotyping of cells used for generation of syngeneic murine models. Tumors from the MMTV-PyMT autochthonous model were used to generate inoculum to inject into wild type mice to generate 1E6, 1E5 and 1E4 syngeneic tumor models. Flow cytometry was performed on three separate batches of inoculum, which were used for each unique experiment and are shown as red (inoculum1), blue (inoculum2), and green (inoculum3). a. Gating strategy for flow cytometry b. Immune cell composition as a frequency of immune cell populations out of CD45+ cells. c. Shown are graphs corresponding to Fig. 1e (CD45), Fig. 1g (CD11b) and Fig. 1h (CD3) color coded by inoculum (experimental run). Graphs show mean ±SEM. *P
Databáze: OpenAIRE