Indium-111 myosin-specific antibodies and technetium-99m pyrophosphate in the detection of acute cardiac rejection of transplanted hearts: studies in a heterotopic rat heart model
| Autor: | Keisuke Ueda, Kan Takeda, Norman D. Lafrance, William A. Baumgartner, Hayden T. Ravert, Ursula Scheffel, Ahvie Herskowitz, Bruce A. Reitz, Henry N. Wagner |
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| Rok vydání: | 1991 |
| Předmět: |
Graft Rejection
Male Pathology medicine.medical_specialty Technetium Tc 99m Pyrophosphate Transplantation Heterotopic Isograft Inflammation Pyrophosphate chemistry.chemical_compound Rats Inbred SHR Myosin Organometallic Compounds medicine Animals Transplantation Homologous Radiology Nuclear Medicine and imaging Radionuclide Imaging Peritoneal Cavity biology business.industry Antibodies Monoclonal General Medicine Rats Transplantation Transplantation Isogeneic chemistry Rats Inbred Lew biology.protein Heart Transplantation medicine.symptom Antibody business Technetium-99m Intracellular |
| Zdroj: | European Journal of Nuclear Medicine. 18:461-466 |
| ISSN: | 1619-7089 0340-6997 |
| DOI: | 10.1007/bf00181284 |
| Popis: | 111In-labelled myosin-specific antibodies were evaluated as an indicator of early changes in acute rejection in a rat heart heterotopic transplant model. Uptake of antibodies was measured in allograft and isograft hearts of animals undergoing different regimens of cyclosporine treatment and compared with the uptake of technetium-99m pyrophosphate. The data were correlated with histological estimation of the severity of myocyte necrosis and signs of early rejection (venous cuffing and endocardial inflammation, indicators of perivascular infiltrate and intermyocyte extension, respectively). Myocyte necrosis in transplanted hearts was reflected by increases in technetium-99m pyrophosphate accumulation (r = 0.88) but was poorly correlated with labelled antibody uptake (r = 0.58). There was no positive correlation between the degree of early cardiac rejection and uptake of either of the radiopharmaceuticals: accumulation of the labelled antibodies paradoxically declined with increased histological severity scores, whereas that of technetium-99m pyrophosphate remained unchanged. Cyclosporine treatment augmented the uptake of labelled antibodies in transplanted hearts. This may be due to alterations in plasma membrane permeability brought about by the drug, resulting in a rise in antibody binding to intracellular myosin. |
| Databáze: | OpenAIRE |
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