Quantification of ASFV DNA and RNA in Ornithodoros Soft Ticks

Autor:	Pereira De Oliveira, Rémi, Vial, Laurence, Le Potier, Marie-Frédérique
Rok vydání:	2022
Zdroj:	African Swine Fever Virus ISBN: 9781071623329 African swine fever virus: Methods and protocols
DOI:	10.1007/978-1-0716-2333-6_7
Popis:	Molecular biology methods are highly sensitive to detect the genome of pathogens and to study their biology. Polymerase chain reaction (PCR) and reverse transcription followed by a polymerase chain reaction (RT-PCR) permit the detection of the presence and the replication of African swine fever virus in soft ticks. Here, we described our techniques to detect and quantify DNA and RNA of African swine fever virus in soft ticks including a housekeeping gene of soft ticks as internal control.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::67c11251dc3780172d9f990d10826e54 https://doi.org/10.1007/978-1-0716-2333-6_7 Zobrazit plný text záznamu