| Popis: |
Supplementary Materials and Methods: Designed antibodies, Expression and Purification, Affinity Measurement, ELISA, Pharmacokinetics, Generation of drug-resistant cell line in vitro, Generation of drug-resistant cell line In vivo, Primers for real-time quantitative PCR, siRNA transfection, Real-time polymerase chain reaction (PCR), Determination of receptor level. Figure S1. DVD-Ig like, tetra-specific, tetravalent antibody CRTB6 binds to antigens. Figure S2. Characterization of Antitumor Activity of bispecific antibodies in vitro and in vivo. Table S1. Selected analytical and affinity data for the parental and four-in-one antibodies. Table S2. Pharmacokinetic parameters of recombinant antibodies in mice. Table S3. Selected analytical and affinity data for the investigated compounds. Table S4. Comparison of cancer cell lines for surface receptor level. |
