Ancient Heavy Metal Contamination in Soils as a Driver of Tolerant Anthyllis vulneraria Rhizobial Communities
| Autor: | Erika Yashiro, Céline Vidal, Jean Claude Cleyet-Marel, Agnieszka Klonowska, Géraldine Maynaud, Brigitte Brunel, Roba Mohamad, Antoine Le Quéré |
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| Přispěvatelé: | Laboratoire des symbioses tropicales et méditerranéennes (UMR LSTM), Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad)-Université Montpellier 1 (UM1)-Institut de Recherche pour le Développement (IRD)-Institut National de la Recherche Agronomique (INRA)-Université Montpellier 2 - Sciences et Techniques (UM2)-Université de Montpellier (UM)-Institut national d’études supérieures agronomiques de Montpellier (Montpellier SupAgro), Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro), Laboratoire de biotechnologie végétale et biologie moléculaire, Faculté des Sciences, Université Moulay Ismail (UMI), Department of Fundamental Microbiology [Lausanne], Université de Lausanne (UNIL), Agence Nationale de la Recherche Programme Contaminants, Ecosystemes et Sante [CES 2008-12], Ecosystemes et Sante [CES 2008-12], Université de Moulay Ismail (UMI) |
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
symbiotic nitrogen fixation sol metal tolerance analysis Environmental pollution Applied Microbiology and Biotechnology Anthyllis vulneraria taxonomy Plant Microbiology Germany RNA Ribosomal 16S Soil Pollutants Phylogeny Soil Microbiology 2. Zero hunger Aminobacter Ecology biology kidney vetch Mesorhizobium food and beverages Fabaceae symbiosis Biodegradation Environmental fixation des métaux fixation de l'azote PIB-type ATPase Nitrogen fixation France Seasons nodulation gene symbiose multilocus sequence Soil microbiology Biotechnology DNA Bacterial taxonomie 030106 microbiology Anthyllis Mining soil Rhizobia 03 medical and health sciences phytostabilisation Bacterial Proteins Symbiosis Metals Heavy phytostabilization mesorhizobium Sequence Analysis DNA 15. Life on land biology.organism_classification tolérance aux metaux anthyllis vulneraria Rec A Recombinases 030104 developmental biology Agronomy [SDE.BE]Environmental Sciences/Biodiversity and Ecology Acyltransferases Food Science |
| Zdroj: | Applied and Environmental Microbiology Applied and Environmental Microbiology, American Society for Microbiology, 2017, 83 (2), 13 p. ⟨10.1128/aem.01735-16⟩ |
| ISSN: | 1098-5336 0099-2240 |
| DOI: | 10.1128/aem.01735-16 |
| Popis: | Anthyllis vulneraria is a legume associated with nitrogen-fixing rhizobia that together offer an adapted biological material for mine-soil phytostabilization by limiting metal pollution. To find rhizobia associated with Anthyllis at a given site, we evaluated the genetic and phenotypic properties of a collection of 137 rhizobia recovered from soils presenting contrasting metal levels. Zn-Pb mine soils largely contained metal-tolerant rhizobia belonging to Mesorhizobium metallidurans or to another sister metal-tolerant species. All of the metal-tolerant isolates harbored the cadA marker gene (encoding a metal-efflux P IB -type ATPase transporter). In contrast, metal-sensitive strains were taxonomically distinct from metal-tolerant populations and consisted of new Mesorhizobium genospecies. Based on the symbiotic nodA marker, the populations comprise two symbiovar assemblages (potentially related to Anthyllis or Lotus host preferences) according to soil geographic locations but independently of metal content. Multivariate analysis showed that soil Pb and Cd concentrations differentially impacted the rhizobial communities and that a rhizobial community found in one geographically distant site was highly divergent from the others. In conclusion, heavy metal levels in soils drive the taxonomic composition of Anthyllis- associated rhizobial populations according to their metal-tolerance phenotype but not their symbiotic nodA diversity. In addition to heavy metals, local soil physicochemical and topoclimatic conditions also impact the rhizobial beta diversity. Mesorhizobium communities were locally adapted and site specific, and their use is recommended for the success of phytostabilization strategies based on Mesorhizobium -legume vegetation. IMPORTANCE Phytostabilization of toxic mine spoils limits heavy metal dispersion and environmental pollution by establishing a sustainable plant cover. This eco-friendly method is facilitated by the use of selected and adapted cover crop legumes living in symbiosis with rhizobia that can stimulate plant growth naturally through biological nitrogen fixation. We studied microsymbiont partners of a metal-tolerant legume, Anthyllis vulneraria , which is tolerant to very highly metal-polluted soils in mining and nonmining sites. Site-specific rhizobial communities were linked to taxonomic composition and metal tolerance capacity. The rhizobial species Mesorhizobium metallidurans was dominant in all Zn-Pb mines but one. It was not detected in unpolluted sites where other distinct Mesorhizobium species occur. Given the different soil conditions at the respective mining sites, including their heavy-metal contamination, revegetation strategies based on rhizobia adapting to local conditions are more likely to succeed over the long term compared to strategies based on introducing less-well-adapted strains. |
| Databáze: | OpenAIRE |
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