Study of acute biochemical effects of thallium toxicity in mouse urine by NMR spectroscopy
| Autor: | M. Memita Devi, Poonam Rana, Deepak Bhatnagar, Shubhra Chaturvedi, Subash Khushu, Ritu Tyagi, Rajendra P. Tripathi, Ahmad Raza Khan |
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| Rok vydání: | 2011 |
| Předmět: |
Male
Taurine Magnetic Resonance Spectroscopy Mice Inbred A Metabolite Protein metabolism chemistry.chemical_element Urine Toxicology Choline Methylamines Mice chemistry.chemical_compound Metals Heavy Animals Metabolomics Thallium Principal Component Analysis 3-Hydroxybutyric Acid Hippurates Poisoning Nuclear magnetic resonance spectroscopy Heavy Metal Poisoning chemistry Biochemistry Creatinine Toxicity |
| Zdroj: | Journal of Applied Toxicology. 31:663-670 |
| ISSN: | 0260-437X |
| DOI: | 10.1002/jat.1617 |
| Popis: | Thallium (Tl) is a toxic heavy metal and its exposure to the human body causes physiological and biochemical changes due to its interference with potassium-dependent biological reactions. A high-resolution 1H NMR spectroscopy based metabonomic approach has been applied for investigating acute biochemical effects caused by thallium sulfate (Tl2SO4). Male strain A mice were divided in three groups and received three doses of Tl2SO4 (5, 10 and 20 mg kg−1 b.w., i.p.). Urine samples collected at 3, 24, 72 and 96 h post-dose time points were analyzed by 1H NMR spectroscopy. NMR spectral data were processed and analyzed using principal components analysis to represent biochemical variations induced by Tl2SO4. Results showed Tl-exposed mice urine to have distinct metabonomic phenotypes and revealed dose- and time-dependent clustering of treated groups. The metabolic signature of urine analysis from Tl2SO4-treated animals exhibited an increase in the levels of creatinine, taurine, hippurate and β-hydroxybutyrate along with a decrease in energy metabolites trimethylamine and choline. These findings revealed Tl-induced disturbed gut flora, membrane metabolite, energy and protein metabolism, representing physiological dysfunction of vital organs. The present study indicates the great potential of NMR-based metabonomics in mapping metabolic response for toxicology, which could ultimately lead to identification of potential markers for Tl toxicity. Copyright © 2011 John Wiley & Sons, Ltd. |
| Databáze: | OpenAIRE |
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