Bone Morphogenetic Protein-2 Conjugated to Quantum Dot®s is Biologically Functional
| Autor: | Vrathasha Vrathasha, Anja Nohe, Daniel Halloran, Hilary W Durbano |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
Fluorescence-lifetime imaging microscopy
General Chemical Engineering medicine.medical_treatment Conjugated system Bone morphogenetic protein 2 lcsh:Chemistry 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine fluorescence imaging BMP-2 medicine General Materials Science mineralization Fluorescein isothiocyanate 030304 developmental biology 0303 health sciences Mathematics::Commutative Algebra Growth factor Condensed Matter::Mesoscopic Systems and Quantum Hall Effect Photobleaching Fluorescence Bone: QDot®s Mathematics::Logic lcsh:QD1-999 chemistry FTIR Quantum dot Biophysics 030217 neurology & neurosurgery Computer Science::Formal Languages and Automata Theory |
| Zdroj: | Nanomaterials Volume 10 Issue 6 Nanomaterials, Vol 10, Iss 1208, p 1208 (2020) |
| ISSN: | 2079-4991 |
| DOI: | 10.3390/nano10061208 |
| Popis: | Quantum Dot® s (QDot® s) are novel, semi-conductive nanostructures that emit a certain fluorescence when excited by specific wavelengths. QDot® s are more photostable, brighter, and photobleach less than other fluorescent dyes. These characteristics give them the potential to be used in many biological applications. The shells of QDot® s are coated with functional groups, such as carboxylate and organic groups, allowing them to couple to peptides/proteins and be used for real-time imaging and high-resolution microscopy. Here, we utilize Quantum Dot® s and Bone Morphogenetic Protein-2 (BMP-2) to create a BMP-2-QDot® s conjugate. BMP-2 is a growth factor that drives many processes such as cardiogenesis, neural growth, and osteogenesis. Despite its numerous roles, the trafficking and uptake of BMP-2 into cells is not well-established, especially during progression of diseases. The results presented here demonstrate for the first time a fluorescent BMP-2 analog that binds to the BMP-receptors (BMPRs), remains biologically active, and is stable for long time periods. Previous attempts to develop a biological BMP-2 analog with Fluorescein isothiocyanate (FITC) or nanodiamonds lacked data on the analog&rsquo s stability. Furthermore, these analogs did not address whether they can signal within the cell by binding to the BMPRs or were mediated by non-stable conjugates. |
| Databáze: | OpenAIRE |
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