All-plastic, miniature, digital fluorescence microscope for three part white blood cell differential measurements at the point of care
Autor: | Rebecca Richards-Kortum, Michal E. Pawlowski, Alessandra Forcucci, Tomasz S. Tkaczyk, Catherine E. Majors |
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Rok vydání: | 2015 |
Předmět: |
Pathology
medicine.medical_specialty Materials science Microscope Acridine orange Article Atomic and Molecular Physics and Optics law.invention chemistry.chemical_compound medicine.anatomical_structure chemistry law White blood cell Fluorescence microscope medicine Biotechnology Biomedical engineering Point of care Whole blood |
Zdroj: | Biomedical Optics Express. 6:4433 |
ISSN: | 2156-7085 |
Popis: | Three-part differential white blood cell counts are used for disease diagnosis and monitoring at the point-of-care. A low-cost, miniature achromatic microscope was fabricated for identification of lymphocytes, monocytes, and granulocytes in samples of whole blood stained with acridine orange. The microscope was manufactured using rapid prototyping techniques of diamond turning and 3D printing and is intended for use at the point-of-care in low-resource settings. The custom-designed microscope requires no manual adjustment between samples and was successfully able to classify three white blood cell types (lymphocytes, granulocytes, and monocytes) using samples of peripheral whole blood stained with acridine orange. |
Databáze: | OpenAIRE |
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