Use of UPLC-ESI-MS/MS to quantitate free amino acid concentrations in micro-samples of mammalian milk
| Autor: | Aurore Martin Agnoux, Marie-Cécile Alexandre-Gouabau, Emmanuelle Desnots, Véronique Ferchaud Roucher, Dominique Darmaun, Clair-Yves Boquien, Charlotte Nael |
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| Přispěvatelé: | Centre de Recherche en Nutrition Humaine, Physiologie des Adaptations Nutritionnelles (PhAN), Institut National de la Recherche Agronomique (INRA)-Université de Nantes (UN), Direction de la Recherche Département Partenariats et Innovation ( DHU 2020), Centre hospitalier universitaire de Nantes (CHU Nantes) |
| Rok vydání: | 2013 |
| Předmět: |
Cow milk
030309 nutrition & dietetics Electrospray ionization Free amino acid Human milk Rat milk UPLC-ESI-MS/MS Tandem mass spectrometry Bioinformatics High-performance liquid chromatography 03 medical and health sciences chemistry.chemical_compound Food and Nutrition Sample preparation Derivatization 030304 developmental biology Detection limit chemistry.chemical_classification 0303 health sciences Multidisciplinary Chromatography Research Repeatability Amino acid chemistry Alimentation et Nutrition [SDV.AEN]Life Sciences [q-bio]/Food and Nutrition |
| Zdroj: | SpringerPlus (2), 622. (2013) SpringerPlus SpringerPlus, SpringerOpen, 2013, 2, pp.622. ⟨10.1186/2193-1801-2-622⟩ |
| ISSN: | 2193-1801 |
| DOI: | 10.1186/2193-1801-2-622 |
| Popis: | Region Pays de la Loire (for the creation of the "Human milk" biocollection at Nantes University Hospital); International audience; Although free amino acids (FAA) account for a small fraction of total nitrogen in mammalian milk, they are more abundant in human milk than in most formulas, and may serve as a readily available source of amino acids for protein synthesis, as well as fulfill specific physiologic roles. We used reversed phase Ultra Performance Liquid Chromatography (UPLC) coupled to electrospray ionization tandem mass spectrometry (ESI-MS/MS) technique for FAA profiling in milks from three species (human, rat and cow) with a simple and rapid sample preparation. The derivatization procedure chosen, combined with UPLC-ESI-MS/MS allowed the quantitation of 21 FAA using labeled amino acids (Internal Standards) over a 10 min run time in micro-samples of mammalian milk (50 μL). The low limit of quantitation was 0.05 pmol/μL for most FAA with good repeatability and reproducibility (mean CV of 5.1%). Higher levels of total FAA were found in human (3032 μM) and rat milk (3460 μM) than in bovine milk (240 μM), with wide differences in the abundances of specific FAA between species. This robust analytical method could be applied to monitor FAA profile in human breast milk, and open the way to individualized adjustment of FAA content for the nutritional management of infants. |
| Databáze: | OpenAIRE |
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