A Natural Combination Extract of Viscum album L. Containing Both Triterpene Acids and Lectins Is Highly Effective against AML In Vivo
| Autor: | Sebastian Jäger, Catharina I. Delebinski, Angelika Eggert, Florian Hoff, Katharina Mulsow, Georg Seifert, Monika Twardziok, Jana Rolff, Susann Kleinsimon |
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| Rok vydání: | 2015 |
| Předmět: |
Male
Adolescent Viscum album lcsh:Medicine Apoptosis HL-60 Cells Mice SCID Inhibitor of apoptosis 600 Technik Medizin angewandte Wissenschaften::610 Medizin und Gesundheit chemistry.chemical_compound Triterpene In vivo Mice Inbred NOD Antineoplastic Combined Chemotherapy Protocols Tumor Cells Cultured Animals Humans Oleanolic Acid lcsh:Science Child Oleanolic acid Cell Proliferation chemistry.chemical_classification Mice Knockout Multidisciplinary biology Dose-Response Relationship Drug Plant Extracts lcsh:R U937 Cells biology.organism_classification Xenograft Model Antitumor Assays In vitro Triterpenes Tumor Burden Leukemia Myeloid Acute chemistry Biochemistry Viscum lcsh:Q Female Plant Lectins Ex vivo Interleukin Receptor Common gamma Subunit Research Article |
| Zdroj: | PLoS ONE PLoS ONE, Vol 10, Iss 8, p e0133892 (2015) |
| DOI: | 10.17169/refubium-18688 |
| Popis: | Aqueous Viscum album L. extracts are widely used in complementary cancer medicine. Hydrophobic triterpene acids also possess anti-cancer properties, but due to their low solubility they do not occur in significant amounts in aqueous extracts. Using cyclodextrins we solubilised mistletoe triterpenes (mainly oleanolic acid) and investigated the effect of a mistletoe whole plant extract on human acute myeloid leukaemia cells in vitro, ex vivo and in vivo. Single Viscum album L. extracts containing only solubilised triterpene acids (TT) or lectins (viscum) inhibited cell proliferation and induced apoptosis in a dose-dependent manner in vitro and ex vivo. The combination of viscum and TT extracts (viscumTT) enhanced the induction of apoptosis synergistically. The experiments demonstrated that all three extracts are able to induce apoptosis via caspase-8 and -9 dependent pathways with down-regulation of members of the inhibitor of apoptosis and Bcl-2 families of proteins. Finally, the acute myeloid leukaemia mouse model experiment confirmed the therapeutic effectiveness of viscumTT-treatment resulting in significant tumour weight reduction, comparable to the effect in cytarabine-treated mice. These results suggest that the combination viscumTT may have a potential therapeutic value for the treatment AML. |
| Databáze: | OpenAIRE |
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