CRL-1072 Enhances Antimycobacterial Activity of Human Macrophages Through Interleukin-8
| Autor: | Chinnaswamy Jagannath, Robert L. Hunter, Suresh Pai, Jeffrey K. Actor |
|---|---|
| Rok vydání: | 1999 |
| Předmět: |
Neutrophils
medicine.drug_class Immunology Microbial Sensitivity Tests Poloxamer Antimycobacterial Monocytes Microbiology Surface-Active Agents Virology medicine Humans Macrophage Interleukin 8 Receptor Peroxidase biology U937 cell Tumor Necrosis Factor-alpha Macrophages Interleukin-8 Granulocyte-Macrophage Colony-Stimulating Factor Hydrogen Peroxide U937 Cells Cell Biology Granulocyte macrophage colony-stimulating factor Myeloperoxidase biology.protein Tumor necrosis factor alpha Mycobacterium avium medicine.drug |
| Zdroj: | Journal of Interferon & Cytokine Research. 19:67-76 |
| ISSN: | 1557-7465 1079-9907 |
| DOI: | 10.1089/107999099314432 |
| Popis: | CRL-1072 is a poloxamer surfactant that kills mycobacteria more effectively within macrophages than in broth cultures. Human macrophages treated with CRL-1072 synthesized interleukin-8 (IL-8), tumor necrosis factor-alpha (TNF-alpha), and granulocyte-macrophage colony-stimulating factor (GM-CSF) in a dose-dependent manner. About 3000 pg of IL-8 per million human macrophages accumulated in cultures treated with 100-1500 ng of poloxamer, with mRNA message for IL-8 induced as early as 2 h. As macrophages do not have IL-RA receptors, a transwell culture was used to study the chemotactic and activating effects of IL-8 between CRL-1072-treated human macrophage effectors and polymorphonuclear neutrophil (PMN) targets. PMN were activated by IL-8 and secreted hydrogen peroxide and myeloperoxidase (MPO). MPO derived from PMN, in turn, activated monocytes for an enhanced killing of intracellular Mycobacterium avium. The ability of CRL-1072 to modulate macrophage-mediated activation of neutrophils and receive a feedback activation signal may form one mechanism by which its antimycobacterial activity is achieved in vivo. |
| Databáze: | OpenAIRE |
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