H7N9 influenza split vaccine with SWE oil-in-water adjuvant greatly enhances cross-reactive humoral immunity and protection against severe pneumonia in ferrets
| Autor: | Paul J.M. Roholl, Nicolas Collin, Harry van Dijken, Martin Friede, Paulo Lee Ho, José A Ferreira, Femke de Heij, Rineke de Jong, Eduardo Alfredo Adami, Sanne Spijkers, Milena Apetito Akamatsu, Justin Mouthaan, Willem Luytjes, Livia Brunner, Jørgen de Jonge |
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| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
lcsh:Immunologic diseases. Allergy Influenza vaccine medicine.medical_treatment Immunology Adaptive immunity lcsh:RC254-282 Article 03 medical and health sciences 0302 clinical medicine Immunity Pandemic medicine Life Science Pharmacology (medical) 030212 general & internal medicine Pharmacology Vaccines business.industry medicine.disease Acquired immune system lcsh:Neoplasms. Tumors. Oncology. Including cancer and carcinogens Virology Virology & Molecular Biology Virologie & Moleculaire Biologie Pneumonia 030104 developmental biology Viral replication Humoral immunity Infectious diseases business Infection lcsh:RC581-607 Adjuvant |
| Zdroj: | npj Vaccines, Vol 5, Iss 1, Pp 1-14 (2020) npj Vaccines 5 (2020) 1 NPJ Vaccines npj Vaccines, 5(1) |
| ISSN: | 2059-0105 |
| Popis: | Until universal influenza vaccines become available, pandemic preparedness should include developing classical vaccines against potential pandemic influenza subtypes. We here show that addition of SWE adjuvant, a squalene-in-water emulsion, to H7N9 split influenza vaccine clearly enhanced functional antibody responses in ferrets. These were cross-reactive against H7N9 strains from different lineages and newly emerged H7N9 variants. Both vaccine formulations protected in almost all cases against severe pneumonia induced by intratracheal infection of ferrets with H7N9 influenza; however, the SWE adjuvant enhanced protection against virus replication and disease. Correlation analysis and curve fitting showed that both VN- and NI-titers were better predictors for protection than HI-titers. Moreover, we show that novel algorithms can assist in better interpretation of large data sets generated in preclinical studies. Cluster analysis showed that the adjuvanted vaccine results in robust immunity and protection, whereas the response to the non-adjuvanted vaccine is heterogeneous, such that the protection balance may be more easily tipped toward severe disease. Finally, cluster analysis indicated that the dose-sparing capacity of the adjuvant is at least a factor six, which greatly increases vaccine availability in a pandemic situation. |
| Databáze: | OpenAIRE |
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