Urinary Biomarkers of Oxidative Status in a Clinical Model of Oxidative Assault
| Autor: | David S. Millington, Ivan Spasojevic, Jeffrey R. Marks, Adviye A. Tolun, Sarah P. Young, Richard T. DiGiulio, Karel Base, P. Kelly Marcom, Frances Wang, Gabriel Mixon, Dora Il'yasova |
|---|---|
| Rok vydání: | 2010 |
| Předmět: |
Adult
medicine.medical_specialty Adolescent Free Radicals Cyclophosphamide Epidemiology medicine.medical_treatment Urinary system Breast Neoplasms Urine medicine.disease_cause Gastroenterology Article Mass Spectrometry Young Adult chemistry.chemical_compound Allantoin Internal medicine Antineoplastic Combined Chemotherapy Protocols Biomarkers Tumor medicine Humans F2-Isoprostanes Chemotherapy business.industry Cancer Middle Aged medicine.disease Oxidative Stress Endocrinology Oncology chemistry Doxorubicin Biomarker (medicine) Female business Oxidative stress Chromatography Liquid medicine.drug |
| Zdroj: | Cancer Epidemiology, Biomarkers & Prevention. 19:1506-1510 |
| ISSN: | 1538-7755 1055-9965 |
| Popis: | Background: We used doxorubicin-based chemotherapy as a clinical model of oxidative assault in humans. Methods: The study recruited newly diagnosed breast cancer patients (n = 23). Urine samples were collected immediately before (T0) and at 1 hour (T1) and 24 hours (T24) after i.v. administration of treatment. Measurements included allantoin and the isoprostanes iPF(2α)-III, iPF(2α)-VI, and 8,12-iso-iPF(2α)-VI along with the prostaglandin 2,3-dinor-iPF(2α)-III, a metabolite of iPF(2α)-III. All biomarkers were quantified using liquid chromatography–tandem mass spectrometry. Results: In all subjects, the levels of the biomarkers increased at T1: allantoin by 22% (P = 0.06), iPF(2α)-III by 62% (P < 0.05), iPF(2α)-VI by 41% (P < 0.05), 8,12-iso-iPF(2α)-VI by 58% (P < 0.05), and 2,3-dinor-iPF(2α)-III by 52% (P < 0.05). At T24, the F2-isoprostanes returned to their baseline levels; the levels of allantoin continued to increase, although the T24-T0 difference was not statistically significant. Conclusions: These results indicate that urinary F2-isoprostanes are valid biomarkers and allantoin is a promising biomarker of oxidative status in humans. Impact: The levels of biomarkers change quickly in response to oxidative assault and can be used to monitor oxidative status in humans in response to treatments related either to generation of free radicals (chemotherapy and radiation therapy) or to antioxidants (inborn metabolic diseases and Down syndrome). Cancer Epidemiol Biomarkers Prev; 19(6); 1506–10. ©2010 AACR. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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