The Cerebellum Harbors a Circadian Oscillator Involved in Food Anticipation
| Autor: | Paul Pévet, Marie-Paule Felder-Schmittbuhl, Jorge E. Mendoza, Etienne Challet, Yannick Bailly |
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| Přispěvatelé: | Institut des Neurosciences Cellulaires et Intégratives (INCI), Université de Strasbourg (UNISTRA)-Centre National de la Recherche Scientifique (CNRS) |
| Jazyk: | angličtina |
| Rok vydání: | 2010 |
| Předmět: |
Male
medicine.medical_specialty Cerebellum [SDV]Life Sciences [q-bio] Circadian clock Central nervous system CLOCK Proteins Motor Activity Biology Mice Purkinje Cells 03 medical and health sciences 0302 clinical medicine Internal medicine medicine Animals Circadian rhythm Luciferases ComputingMilieux_MISCELLANEOUS 030304 developmental biology 2. Zero hunger Mice Inbred C3H 0303 health sciences Appetite Regulation General Neuroscience Feeding Behavior Period Circadian Proteins Articles Immunohistochemistry Circadian Rhythm Rats Motor coordination Mice Inbred C57BL CLOCK Endocrinology medicine.anatomical_structure Mutation Nuclear Receptor Subfamily 1 Group D Member 1 Rats Transgenic 030217 neurology & neurosurgery PER1 GRID2 |
| Zdroj: | Journal of Neuroscience Journal of Neuroscience, Society for Neuroscience, 2010, 30 (5), pp.1894-1904. ⟨10.1523/JNEUROSCI.5855-09.2010⟩ |
| ISSN: | 0270-6474 1529-2401 |
| Popis: | The cerebellum participates in motor coordination as well as in numerous cerebral processes, including temporal discrimination. Animals can predict daily timing of food availability, as manifested by food-anticipatory activity under restricted feeding. By studyingex vivoclock gene expression byin situhybridization and recordingin vitro Per1-luciferasebioluminescence, we report that the cerebellum contains a circadian oscillator sensitive to feeding cues (i.e., whose clock gene oscillations are shifted in response to restricted feeding). Food-anticipatory activity was markedly reduced in mice injected intracerebroventricularly with an immunotoxin that depletes Purkinje cells (i.e., OX7-saporin). Mice bearing the hotfoot mutation (i.e.,Grid2ho/ho) have impaired cerebellar circuitry and mild ataxic phenotype.Grid2ho/homice fedad libitumshowed regular behavioral rhythms and day–night variations of clock gene expression in the hypothalamus and cerebellum. When challenged with restricted feeding, however,Grid2ho/homice did not show any food-anticipatory rhythms, nor timed feeding-induced changes in cerebellar clock gene expression. In hypothalamic arcuate and dorsomedial nuclei, however, shifts inPer1expression in response to restricted feeding were similar in cerebellar mutant and wild-type mice. Furthermore, plasma corticosterone and metabolites before mealtime did not differ between cerebellar mutant and wild-type mice. Together, these data define a role for the cerebellum in the circadian timing network and indicate that the cerebellar oscillator is required for anticipation of mealtime. |
| Databáze: | OpenAIRE |
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