Pneumocystis carinii induces an oxidative burst in alveolar macrophages
| Autor: | R. J. Helmke, John A Mangos, Humberto A. Hidalgo, Victor F. German |
|---|---|
| Rok vydání: | 1992 |
| Předmět: |
Male
Phagocytosis Immunology Dose-Response Relationship Immunologic Biology Microbiology chemistry.chemical_compound parasitic diseases Freezing Macrophages Alveolar Macrophage Animals Respiratory Burst Pneumocystis Pneumonia Pneumocystis Zymosan Rats Inbred Strains Hydrogen Peroxide Opsonin Proteins Molecular biology In vitro Respiratory burst respiratory tract diseases Rats Antibody opsonization Microscopy Electron Infectious Diseases Pneumocystis carinii chemistry Cell culture Parasitology Research Article |
| Zdroj: | Infection and immunity. 60(1) |
| ISSN: | 0019-9567 |
| Popis: | There is evidence that alveolar macrophages (AM) play a role in the clearing of Pneumocystis carinii from the lungs. To investigate the mechanisms involved in this process, we studied in vitro the induction of an oxidative burst by P. carinii in a cell line of macrophages (NR8383) and AM from normal rats. P. carinii was added to macrophage monolayers (10(6) cells), and the H2O2 produced after 4 h of incubation was measured. Both NR8383 macrophages and normal rat AM produced H2O2 in response to P. carinii cysts and trophozoites isolated from dexamethasone-treated rats, although the amount of H2O2 induced in AM from normal rats was larger. NR8383 macrophages bound and phagocytized both P. carinii cysts and trophozoites and produced increasing amounts of H2O2 as a dose-related response to cysts and trophozoites. Opsonization of P. carinii with normal rat serum increased H2O2 production by both types of macrophages; this enhancement was decreased, but not abolished, when the serum was first depleted of complement by heat treatment. These findings demonstrate that NR8383 macrophages and normal rat AM produce an oxidative burst in response to P. carinii and that this response is enhanced by complement. |
| Databáze: | OpenAIRE |
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