Enzyme-linked immunosorbent assay detection of microcystins using new monoclonal antibodies

Autor:	Dongjin Pyo, Euiyule Choi, Jungae Lee
Rok vydání:	2004
Předmět:	Microcystis Microcystins medicine.drug_class Clinical Biochemistry Immunology Bacterial Toxins Enzyme-Linked Immunosorbent Assay Monoclonal antibody medicine.disease_cause Peptides Cyclic Sensitivity and Specificity Bacterial Proteins polycyclic compounds medicine Immunology and Allergy Microcystis aeruginosa Solid phase extraction Detection limit Chromatography biology Toxin Chemistry Hepatotoxin Antibodies Monoclonal Water biology.organism_classification Molecular biology Orders of magnitude (mass) Medical Laboratory Technology Marine Toxins
Zdroj:	Journal of immunoassayimmunochemistry. 25(3)
ISSN:	1532-1819
Popis:	New monoclonal antibodies (mAbs) against the microcystin-leucine-arginine variant (microcystin-LR), a cyclic peptide toxin of the freshwater cyanobacterium Microcystis aeruginosa, were prepared from cloned hybridoma cell lines. Using these mAbs, an enzyme-linked immunosorbent assay (ELISA) experiment was made for the detection of cyanobacterial hepatotoxins, microcystins in water sampled in Soyang Lake, Korea. The performance of the ELISA test with mAbs established in this study was evaluated. The ELISA detection was compared with HPLC detection. Since the detection limit of HPLC is several orders of magnitude higher than with ELISA, attention was also paid to concentration of samples with solid phase extraction cartridges.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::67a5577c25f5728c5083113099a4a857 https://pubmed.ncbi.nlm.nih.gov/15461385 Zobrazit plný text záznamu Plný text ve formátu PDF Plný text ve formátu HTML
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