Combined therapy with flk1-based DNA vaccine and interleukin-12 results in enhanced antiangiogenic and antitumor effects
| Autor: | Hui Qiu, Jian Chen, Ji-xiao Liu, Ke-ke Feng, Hongyang Zhao |
|---|---|
| Rok vydání: | 2004 |
| Předmět: |
Salmonella typhimurium
Cancer Research Salmonella Biology medicine.disease_cause Cancer Vaccines DNA vaccination chemistry.chemical_compound Mice Splenocyte medicine Tumor Cells Cultured Vaccines DNA Animals Vector (molecular biology) Gene Neovascularization Pathologic Glioma Cytotoxicity Tests Immunologic Molecular biology Interleukin-12 Vascular Endothelial Growth Factor Receptor-2 Vascular endothelial growth factor Mice Inbred C57BL CTL Oncology chemistry Cancer research Interleukin 12 |
| Zdroj: | Cancer letters. 221(1) |
| ISSN: | 0304-3835 |
| Popis: | In this study, we investigated the anti-vasculature effects and the antitumor effects of combining attenuated Salmonella typhimurium vaccine strain encoding murine vascular endothelial growth factor (VEGF) receptor-2 (flk1) with plasmid DNA vector encoding the murine interleukin-12 (mIL-12) gene. In combination, flk1 based DNA vaccine and mIL-12 slowed down tumor growth more effectively than either one alone. Splenocytes from the combined group were showed a strong CTL response against both the flk1 and tumor cells. Automated image analysis revealed that the mean microvessel density was significantly reduced after administering either flk1 based DNA vaccine or mIL-12. In addition, the combination of flk1 based DNA vaccine and mIL-12 appeared more effective at reducing the microvessel density of tumor (P |
| Databáze: | OpenAIRE |
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