Development of flocculent Saccharomyces cerevisiae strain GYK-10 for the selective fermentation of glucose/fructose in sugar mills
| Autor: | Satoshi Ohara, Akira Sugimoto, Takayuki Masuda, Takaomi Yasuhara, Yasuhiro Fukushima, Hiromi Yamagishi, Taku Kato |
|---|---|
| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Sucrose 030106 microbiology Saccharomyces cerevisiae Bioengineering Fructose Haploidy Applied Microbiology and Biotechnology 03 medical and health sciences chemistry.chemical_compound Bioreactors Food Industry Ethanol fuel Food science Sugar chemistry.chemical_classification Ethanol biology Flocculation food and beverages biology.organism_classification Diploidy Yeast Saccharum Reducing sugar Glucose 030104 developmental biology chemistry Biochemistry Fermentation Biotechnology |
| Zdroj: | Journal of Bioscience and Bioengineering. 122:58-63 |
| ISSN: | 1389-1723 |
| Popis: | Advances in glucose/fructose-selective ethanol production have successfully enhanced raw sugar extraction from sugarcane juice by converting inhibitory substances (i.e., glucose/fructose) into ethanol, which is removed by subsequent operations in cane sugar mills. However, the commercial implementation of this breakthrough process in existing cane sugar mills requires a yeast strain that (i) can be used in food production processes, (ii) exhibits stable saccharometabolic selectivity, and (iii) can be easily separated from the saccharide solution. In this study, we developed a suitable saccharometabolism-selective and flocculent strain, Saccharomyces cerevisiae GYK-10. We obtained a suitable yeast strain for selective fermentation in cane sugar mills using a yeast mating system. First, we crossed a haploid strain defective in sucrose utilization with a flocculent haploid strain. Next, we performed tetrad dissection of the resultant hybrid diploid strain and selected GYK-10 from various segregants by investigating the sucrose assimilation and flocculation capacity phenotypes. Ten consecutive fermentation tests of the GYK-10 strain using a bench-scale fermentor confirmed its suitability for the implementation of practical selective fermentation in a commercial sugar mill. The strain exhibited complete saccharometabolic selectivity and sustained flocculation, where it maintained a high ethanol yield and conversion rate throughout the test. |
| Databáze: | OpenAIRE |
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