Decline of antibody response in indirect ELISA tests during the periparturient period caused diagnostic gaps in Coxiella burnetii and BVDV serology in pluriparous cows within a Holstein dairy herd
| Autor: | V. Zoche-Golob, J. Weber, Markus Freick, J. Walraph |
|---|---|
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
040301 veterinary sciences Ice calving Cattle Diseases Enzyme-Linked Immunosorbent Assay Antibodies Viral Serology 0403 veterinary science 03 medical and health sciences Blood serum Animal science Pregnancy Lactation medicine Animals Longitudinal Studies Diarrhea Viruses Bovine Viral General Veterinary biology food and beverages 04 agricultural and veterinary sciences Coxiella burnetii biology.organism_classification Antibodies Bacterial Bovine herpesvirus 1 030104 developmental biology medicine.anatomical_structure Milk Antibody Formation Herd Colostrum Cattle Female |
| Zdroj: | Research in veterinary science. 118 |
| ISSN: | 1532-2661 |
| Popis: | In cattle, indirect enzyme-linked immunosorbent assay (ELISA) tests are commonly used in serological routine diagnostics. In a longitudinal study design, changes in relative optical density (OD) from drying-off until week 11 after calving were analyzed in blood and milk samples from pluriparous dairy cows (n=21) using a commercial indirect anti-C. burnetii ELISA test. In a second part of this study, changes over time were evaluated in blood and milk samples of 11 of these cows in an indirect ELISA detecting anti-Bovine viral diarrhea virus (BVDV) antibodies. Regarding the cows which were still in the herd at the time of calving, blood serological qualitative changes from positive to negative or indeterminate were demonstrated in 7/20 cows (35%) for the anti-C. burnetii indirect ELISA and in 2/10 cows (20%) for the anti-BVDV indirect ELISA, respectively, during the period from 14days ante partum to calving. Relative OD in the anti-C. burnetii and the anti-BVDV indirect ELISA tests followed basically similar courses over time. In blood serum, relative OD initially increased after drying-off, before a drop around calving was observed. After the colostrum period, relative OD in blood serum showed an increase until week 11 of lactation. In colostrum samples, relative OD levels were higher than in milk samples obtained one day before drying-off. After parturition, relative OD in milk decreased until week 6 of lactation in the anti-C. burnetii indirect ELISA and until week 11 in the anti-BVDV indirect ELISA, respectively. In conclusion, blood serological investigations in periparturient dairy cows using indirect ELISA kits should be avoided. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full Text from ScienceDirect