Periodontal pocket as a potential reservoir of high risk human papilloma virus: A pilot study
| Autor: | Manjunath Mundoor Dayakar, Dinesh Gupta, Anna Shipilova |
|---|---|
| Rok vydání: | 2015 |
| Předmět: |
Pathology
medicine.medical_specialty Gingival and periodontal pocket In situ hybridization 03 medical and health sciences 0302 clinical medicine human papilloma virus medicine Gingival sulcus Periodontitis E6/E7 messenger RNA business.industry virus diseases 030206 dentistry Periodontium medicine.disease head and neck carcinoma Chronic periodontitis Head and neck squamous-cell carcinoma periodontal pocket lcsh:RK1-715 lcsh:Dentistry 030220 oncology & carcinogenesis Periodontics Papilloma Original Article business |
| Zdroj: | Journal of Indian Society of Periodontology, Vol 20, Iss 2, Pp 136-140 (2016) Journal of Indian Society of Periodontology |
| ISSN: | 0972-124X |
| DOI: | 10.4103/0972-124x.170815 |
| Popis: | Aim: Human papilloma viruses (HPVs) are small DNA viruses that have been identified in periodontal pocket as well as gingival sulcus. High risk HPVs are also associated with a subset of head and neck carcinomas. HPV detection in periodontium has previously involved DNA detection. This study attempts to: (a) Detect the presence or absence of high risk HPV in marginal periodontiun by identifying E6/E7 messenger RNA (mRNA) in cells from samples obtained by periodontal pocket scraping. (b) Detect the percentage of HPV E6/E7 mRNA in cells of pocket scrapings, which is responsible for producing oncoproteins E6 and E7. Materials and Methods: Pocket scrapings from the periodontal pockets of eight subjects with generalized chronic periodontitis were taken the detection of presence or absence of E6, E7 mRNA was performed using in situ hybridization and flow cytometry. Results: HPV E6/E7 mRNA was detected in four of the eight samples. Conclusion: Presence of high risk human papillomaviruses in periodontal pockets patients of diagnosed with chronic periodontitis, not suffering from head and neck squamous cell carcinoma in the present day could link periodontitis to HPV related squamous cell carcinoma. Prevalence studies are needed detecting the presence of HPV in marginal periodontium as well as prospective studies of HPV positive periodontitis patients are required to explore this possible link. |
| Databáze: | OpenAIRE |
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