Single nucleotide polymorphisms of the genes IL-2, IL-2RB, and JAK3 in patients with cutaneous leishmaniasis caused by Leishmania (V.) guyanensis in Manaus, Amazonas, Brazil

Autor: Felipe Jules de Araújo Santos, Tirza Gabrielle Ramos de Mesquita, Rajendranath Ramasawmy, José do Espírito Santo Junior, Moacir Couto de Andrade Júnior, Sinésio Talhari, Mara Lúcia Gomes de Souza, Lener Santos da Silva
Jazyk: angličtina
Rok vydání: 2019
Předmět:
Leishmaniasis
Mucocutaneous
Male
Heredity
Intron
Polymerase Chain Reaction
Geographical locations
Il2 Protein
Human
0302 clinical medicine
Leishmaniasis
Protozoans
Innate Immune System
Eukaryota
American Indian
Cytokines
Medicine
Jak3 Gene
Polymorphism
Restriction Fragment Length
Human
Il 2 Gene
Genotype
Science
Leishmania guyanensis
Immunology
Endemic Disease
Single-nucleotide polymorphism
Il 2rb Gene
Molecular Genetics
03 medical and health sciences
Genetics
Humans
Molecular Biology
Brasil
Organisms
Biology and Life Sciences
Janus Kinase 3
Molecular Development
medicine.disease
Tropical Diseases
030104 developmental biology
Jak3 Protein
Mouse
People and places
Developmental Biology
0301 basic medicine
Skin Leishmaniasis
Physiology
Gene Expression
Gene Frequency
Zoonoses
Immune Physiology
Medicine and Health Sciences
Promoter Regions
Genetic
Leishmania
Il2rb Protein
Human
Multidisciplinary
Middle Aged
Clinical Trial
Genetic Predisposition To Disease
Europe
Genetic Mapping
Infectious Diseases
Female
Genetic Association
Brazil
Research Article
Neglected Tropical Diseases
Adult
Major Clinical Study
030231 tropical medicine
Amazonas
Variant Genotypes
Leishmania Guyanensis
Biology
Genetic Predisposition
Polymorphism
Single Nucleotide
Cutaneous leishmaniasis
medicine
Parasitic Diseases
Genetic Predisposition to Disease
Controlled Study
Promoter Region
Interleukin-2 Receptor Beta Subunit
Allele
Gene
Allele frequency
Protozoan Infections
Indians
South American
South America
Molecular biology
Parasitic Protozoans
Introns
Interleukin-2 Receptor beta Subunit
Immune System
Interleukin 2 Receptor Beta
Interleukin-2
Zdroj: PLoS ONE, Vol 14, Iss 8, p e0220572 (2019)
PLoS ONE
Repositório Institucional do INPA
Instituto Nacional de Pesquisas da Amazônia (INPA)
instacron:INPA
ISSN: 1932-6203
Popis: Leishmaniasis is a disease caused by intracellular protozoan parasites of the genus Leishmania. In endemic areas, only a portion of exposed subjects develops cutaneous leishmaniasis (CL), suggesting that the genetic inheritance of the host plays a vital role in both resistance and susceptibility to the disease. Interleukin-2 (IL-2) is a cytokine that plays a central role in the regulation of the immune response in infection through the axis IL-2/IL-2R (receptor) complex, triggering a series of intracellular events, among which the signaling of Janus kinase/signal transducers and activators of transcription (JAK-STAT). The present study aimed at verifying the possible relationship between single nucleotide polymorphism (s) (SNP s) in the genes IL-2, IL-2RB, and JAK3 in subjects with CL caused by Leishmania guyanensis in the city of Manaus, state of Amazonas, Brazil. 820 patients with CL and 850 healthy subjects (control group) coming from the same endemic areas as the patients were examined. The SNPs -2425G/A (rs4833248) and -330 T/G (rs2069762), located in the IL-2 gene promoter region, seem to influence the expression of the gene and the SNP +10558G/A (rs1003694) and +13295T/C (rs3212760) located in the 3rd intron of the IL-2RB gene and the 13th intron of the JAK3 gene, respectively, were studied by PCR-RFLP. Genotypes and alleles frequencies were obtained by direct counting. For the comparison between the two groups, the χ2 test with OR (odds ratio) and the 95% confidence interval (CI) were used. Similar genotypes and alleles frequencies for the different SNPs were observed in both patients with CL and healthy controls. Comparison of genotypic and allelic frequency between patients with CL and healthy subjects did not show any difference. These polymorphisms do not predict susceptibility to, or protection against the development of CL caused by L. guyanensis in the Amazonas. © 2019 de Araújo Santos et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Databáze: OpenAIRE
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