Renewable and tuneable bio-LPG blends derived from amino acids
| Autor: | Nigel S. Scrutton, Mohamed Amer, Michael H. Smith, Robin Hoeven, Matthew Faulkner, Helen S. Toogood, Paul P. Kelly |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
0106 biological sciences
lcsh:Biotechnology Management Monitoring Policy and Law 01 natural sciences Applied Microbiology and Biotechnology lcsh:Fuel Butyric acid Propane 03 medical and health sciences chemistry.chemical_compound lcsh:TP315-360 lcsh:TP248.13-248.65 Manchester Institute of Biotechnology 010608 biotechnology Escherichia coli Organic chemistry 030304 developmental biology Isobutane chemistry.chemical_classification Alkane 0303 health sciences Halomonas biology Renewable Energy Sustainability and the Environment Research Fatty acid Butane ResearchInstitutes_Networks_Beacons/manchester_institute_of_biotechnology biology.organism_classification Microbial pathway engineering Amino acid General Energy chemistry Biofuels Bio-LPG Biotechnology |
| Zdroj: | Amer, M, Hoeven, R, Kelly, P, Faulkner, M, Smith, M H, Toogood, H & Scrutton, N 2020, ' Renewable and Tuneable Bio-LPG Blends Derived from Amino Acids ', Biotechnology for Biofuels . https://doi.org/10.1186/s13068-020-01766-0 Biotechnology for Biofuels, Vol 13, Iss 1, Pp 1-15 (2020) Biotechnology for Biofuels |
| ISSN: | 1754-6834 |
| DOI: | 10.1186/s13068-020-01766-0 |
| Popis: | Background Microbial biorefinery approaches are beginning to define renewable and sustainable routes to clean-burning and non-fossil fuel-derived gaseous alkanes (known as ‘bio-LPG’). The most promising strategies have used a terminal fatty acid photodecarboxylase, enabling light-driven propane production from externally fed waste butyric acid. Use of Halomonas (a robust extremophile microbial chassis) with these pathways has enabled bio-LPG production under non-sterile conditions and using waste biomass as the carbon source. Here, we describe new engineering approaches to produce next-generation pathways that use amino acids as fuel precursors for bio-LPG production (propane, butane and isobutane blends). Results Multiple pathways from the amino acids valine, leucine and isoleucine were designed in E. coli for the production of propane, isobutane and butane, respectively. A branched-chain keto acid decarboxylase-dependent pathway utilising fatty acid photodecarboxylase was the most effective route, generating higher alkane gas titres over alternative routes requiring coenzyme A and/or aldehyde deformylating oxygenase. Isobutane was the major gas produced in standard (mixed amino acid) medium, however valine supplementation led to primarily propane production. Transitioning pathways into Halomonas strain TQ10 enabled fermentative production of mixed alkane gases under non-sterile conditions on simple carbon sources. Chromosomal integration of inducible (~ 180 mg/g cells/day) and constitutive (~ 30 mg/g cells/day) pathways into Halomonas generated production strains shown to be stable for up to 7 days. Conclusions This study highlights new microbial pathways for the production of clean-burning bio-LPG fuels from amino acids. The use of stable Halomonas production strains could lead to gas production in the field under non-sterile conditions following process optimisation. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full text from SpringerLink