Functional modules and expression of mouse p40 phox and p67phox, SH3-domain-containing proteins involved in the phagocyte NADPH oxidase complex
| Autor: | Hideki Sumimoto, Yasuyuki Fukumaki, Kazuhito Mizuki, Kenichiro Hata, Qi-Wen Fan, Kenji Kadomatsu, Koichiro Takeshige, Takashi Ito, Yoshiyuki Sakaki, Yohko Kage |
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| Rok vydání: | 1998 |
| Předmět: |
Transcription
Genetic Phagocyte Molecular Sequence Data Biology Hippocampus Polymerase Chain Reaction Biochemistry SH3 domain src Homology Domains Mice chemistry.chemical_compound NADPH oxidase complex Cerebellum medicine Animals Humans Amino Acid Sequence RNA Messenger Cloning Molecular In Situ Hybridization B cell DNA Primers Gene Library Phagocytes Oxidase test NADPH oxidase Base Sequence Sequence Homology Amino Acid Superoxide Macrophages Brain NADPH Oxidases Signal transducing adaptor protein Phosphoproteins Recombinant Proteins Cell biology Kinetics medicine.anatomical_structure chemistry biology.protein Sequence Alignment |
| Zdroj: | European Journal of Biochemistry. 251:573-582 |
| ISSN: | 1432-1033 0014-2956 |
| Popis: | The phagocyte NADPH oxidase is activated during phagocytosis to produce superoxide, a precursor of microbicidal oxidants. The formation of the active oxidase complex at the membrane requires translocation of the Rac GTPase and two specialized cytosolic proteins that harbor SH3 domains, p67phox and p47phox. Another SH3-domain-containing protein p40phox, which is constitutively associated with p67phox in phagocytes, also enters the complex upon cell stimulation. Here we describe how we cloned mouse cDNAs encoding p40phox and its partner in phagocytes, p67phox. Both p40phox and p67phox comprise several protein-binding modules that are structurally and functionally well conserved between mouse and human, indicating their nature as adaptor proteins. We have also systematically investigated expression of the gene for p40phox in comparison with those for p67phox and p47phox. Distributions of the mRNAs for the three proteins among tissues are similar, with the most abundant expression in the spleen. The messages are abundant not only in phagocytic cells, but also in B cell lineage. The p40phox gene, but not the other two, is expressed in some types of cells such as plasma cells and T lymphocytes. Furthermore, in situ hybridization analysis shows that the p40phox mRNA is distributed in neuronal cells of mouse brain, providing evidence that one of the genes for the specialized oxidase factors is expressed in neurons. These observations raise the possibility that the adaptor protein p40phox plays a heretofore unsuspected role via interacting with other proteins in the cells that do not express p67phox or p47phox. |
| Databáze: | OpenAIRE |
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