Monoclonal Antibodies Against Hepatitis B S Antigen: Production, Characterization, and Use for Diagnosis
| Autor: | Adys Brito, Isis Amores, Aurora Delhanty, Ileana Baluja, Virginia Soto, Julio Ventura, Carmen Acosta, Irina Valdivia |
|---|---|
| Rok vydání: | 2000 |
| Předmět: |
HBsAg
medicine.drug_class Immunology medicine.disease_cause Monoclonal antibody Mice Orthohepadnavirus Antigen Genetics medicine Animals Humans Hepatitis B virus Mice Inbred BALB C Hepatitis B Surface Antigens Hybridomas biology business.industry Antibodies Monoclonal virus diseases Hepatitis B biology.organism_classification Virology Molecular biology digestive system diseases Hepadnaviridae Monoclonal biology.protein Female Antibody business |
| Zdroj: | Hybridoma. 19:259-262 |
| ISSN: | 0272-457X |
| Popis: | Three different hybridoma clones secreting anti-HBsAg antibody were constructed by fusing cells of mouse myeloma line Ag8-X63 with splenocytes from BALB/c mice immunized with recombinant HBsAg and natural HBV. The monoclonal antibodies obtained were characterized immunologically, and two were used to develop UMELISA for detection of HBsAg. This monoclonal assay enabled the detection of 0.1 UPE/mL with reference to the standard of the Paul Ehrlich Institute (Frankfurt, Germany). The assay compared well with a commercially available kit (UMELISA HBsAg) and was used for detection of HBsAg in blood donors. |
| Databáze: | OpenAIRE |
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