Urine metabolomics of rats with chronic atrophic gastritis

Autor: Liang-Kun Zhang, Jian Chen, Xi-Jian Liu, Ling Li, Keyun Sun, Tao Han, Qian-Qian Sun, Hailiang Huang, Guoxiu Zu
Jazyk: angličtina
Rok vydání: 2020
Předmět:
Male
Physiology
Methylnitronitrosoguanidine
Atrophic gastritis
Nitrogen Metabolism
Urine
Biochemistry
Mass Spectrometry
Analytical Chemistry
Pathogenesis
chemistry.chemical_compound
Spectrum Analysis Techniques
Medicine and Health Sciences
Metabolites
Medicine
Amino Acids
Purine metabolism
Liquid Chromatography
Multidisciplinary
Organic Compounds
Chromatographic Techniques
Body Fluids
Chemistry
Physical Sciences
Purine Metabolism
Metabolic Pathways
Anatomy
Basic Amino Acids
Metabolic Networks and Pathways
Research Article
Gastritis
Atrophic
medicine.medical_specialty
Liquid Chromatography-Mass Spectrometry
Science
Research and Analysis Methods
Metabolomics
Internal medicine
Animals
Histidine
Rats
Wistar
business.industry
Organic Chemistry
Chemical Compounds
Biology and Life Sciences
Proteins
Metabolism
medicine.disease
Rats
Amino Acid Metabolism
Disease Models
Animal
Metabolic pathway
Endocrinology
chemistry
business
Biomarkers
Zdroj: PLoS ONE, Vol 15, Iss 11, p e0236203 (2020)
PLoS ONE
ISSN: 1932-6203
Popis: Background/aim To use liquid chromatography-mass spectrometry (LC-MS) to identify endogenous differential metabolites in the urine of rats with chronic atrophic gastritis (CAG). Materials and methods Methylnitronitrosoguanidine (MNNG) was used to produce a CAG model in Wistar rats, and HE staining was used to determine the pathological model. LC-MS was used to detect the differential metabolic profiles in rat urine. Diversified analysis was performed by the statistical method. Results Compared with the control group, the model group had 68 differential metabolites, 25 that were upregulated and 43 that were downregulated. The main metabolic pathways were D-glutamine and D-glutamic acid metabolism, histidine metabolism and purine metabolism. Conclusion By searching for differential metabolites and metabolic pathways in the urine of CAG rats, this study provides effective experimental data for the pathogenesis and clinical diagnosis of CAG.
Databáze: OpenAIRE
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