Expression of bacterial levanase in yeast enables simultaneous saccharification and fermentation of grass juice to bioethanol
| Autor: | Andrew G. S. Warrilow, Josie E. Parker, Colin J. Jackson, S. M. Morris, Steven L. Kelly, Iain Donnison, Carolyn Greig, Nicola J. Rolley, Claire M. Martel, Diane E. Kelly |
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| Rok vydání: | 2011 |
| Předmět: |
Environmental Engineering
Glycoside Hydrolases Levanase Molecular Sequence Data Inulin Saccharomyces cerevisiae Bioengineering Bacillus subtilis Poaceae Microbiology chemistry.chemical_compound Transformation Genetic Bacterial Proteins Amino Acid Sequence Food science Waste Management and Disposal Recombination Genetic Expression vector Ethanol biology Renewable Energy Sustainability and the Environment Fructose General Medicine biology.organism_classification Yeast chemistry Biofuels Fermentation Carbohydrate Metabolism Biotechnology |
| Zdroj: | Bioresource Technology. 102:1503-1508 |
| ISSN: | 0960-8524 |
| Popis: | This study demonstrates use of recombinant yeast to simultaneously saccharify and ferment grass juice (GJ) to bioethanol. A modified Bacillus subtilis levanase gene (sacC) in which the native bacterial signal sequence was replaced with a yeast α-factor domain, was synthesised with yeast codon preferences and transformed into Saccharomyces cerevisiae (strain AH22) using the expression vector pMA91. AH22:psacC transformants secreted sacCp as an active, hyper-glycosylated (>180 kDa) protein allowing them to utilise inulin (β[2-1] linked fructose) and levan (β[2-6] linkages) as growth substrates. The control (AH22:pMA91) strain, transformed with empty plasmid DNA was not able to utilise inulin or levan. When cultured on untreated GJ levels of growth and bioethanol production were significantly higher in experiments with AH22:psacC than with AH22:pMA91. Bioethanol yields from AH22:psacC grown on GJ (32.7[±4] mg mL(-1)) compared closely to those recently achieved (Martel et al., 2010) using enzymatically pre-hydrolysed GJ (36.8[±4] mg mL(-1)). |
| Databáze: | OpenAIRE |
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