Cocaine and Amphetamine Regulated Transcript (CART) Expression Changes in the Stomach Wall Affected by Experimentally Induced Gastric Ulcerations

Autor: Hanna Antushevich, Amelia Franke-Radowiecka, Michal Zalecki, Adrianna Plywacz
Jazyk: angličtina
Rok vydání: 2021
Předmět:
0301 basic medicine
Pathology
Swine
0403 veterinary science
enteric nervous system
immune system diseases
CART
Biology (General)
Spectroscopy
Acetic Acid
Neurons
Gastrointestinal tract
medicine.diagnostic_test
Q-PCR
Stomach
virus diseases
04 agricultural and veterinary sciences
General Medicine
Anti-Bacterial Agents
Computer Science Applications
Chemistry
Real-time polymerase chain reaction
medicine.anatomical_structure
immunohistochemistry
Immunohistochemistry
Female
stomach
Cart
medicine.medical_specialty
040301 veterinary sciences
QH301-705.5
Myenteric Plexus
Nerve Tissue Proteins
Biology
Immunofluorescence
Article
Catalysis
Cocaine and amphetamine regulated transcript
Inorganic Chemistry
03 medical and health sciences
parasitic diseases
medicine
Animals
Stomach Ulcer
Physical and Theoretical Chemistry
Molecular Biology
QD1-999
ulcer
Organic Chemistry
digestive system diseases
030104 developmental biology
nervous system
cocaine- and amphetamine-regulated transcript
Enteric nervous system
Zdroj: International Journal of Molecular Sciences, Vol 22, Iss 7437, p 7437 (2021)
International Journal of Molecular Sciences
Volume 22
Issue 14
ISSN: 1661-6596
1422-0067
Popis: Cocaine- and amphetamine-regulated transcript (CART) is a peptide suggested to play a role in gastrointestinal tract tissue reaction to pathology. Gastric ulceration is a common disorder affecting huge number of people, and additionally, it contributes to the loss of pig livestock production. Importantly, ulceration as a focal disruption affecting deeper layers of the stomach wall differs from other gastrointestinal pathologies and should be studied individually. The pig’s gastrointestinal tract, due to its many similarities to the human counterpart, provides a valuable experimental model for studying digestive system pathologies. To date, the role of CART in gastric ulceration and the expression of the gene encoding CART in porcine gastrointestinal tube are completely unknown. Therefore, we aimed to verify the changes in the CART expression by Q-PCR (gene encoding CART in the tissue) and double immunofluorescence staining combined with confocal microscopy (CART immunofluorescence in enteric nervous system) in the porcine stomach tissues adjacent to gastric ulcerations. Surprisingly, we found that gastric ulcer caused a significant decrease in the expression of CART-encoding gene and huge reduction in the percentage of CART-immunofluorescent myenteric perikarya and neuronal fibers located within the circular muscle layer. Our results indicate a unique CART-dependent gastric response to ulcer disease.
Databáze: OpenAIRE
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