Cocaine and Amphetamine Regulated Transcript (CART) Expression Changes in the Stomach Wall Affected by Experimentally Induced Gastric Ulcerations
Autor: | Hanna Antushevich, Amelia Franke-Radowiecka, Michal Zalecki, Adrianna Plywacz |
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Jazyk: | angličtina |
Rok vydání: | 2021 |
Předmět: |
0301 basic medicine
Pathology Swine 0403 veterinary science enteric nervous system immune system diseases CART Biology (General) Spectroscopy Acetic Acid Neurons Gastrointestinal tract medicine.diagnostic_test Q-PCR Stomach virus diseases 04 agricultural and veterinary sciences General Medicine Anti-Bacterial Agents Computer Science Applications Chemistry Real-time polymerase chain reaction medicine.anatomical_structure immunohistochemistry Immunohistochemistry Female stomach Cart medicine.medical_specialty 040301 veterinary sciences QH301-705.5 Myenteric Plexus Nerve Tissue Proteins Biology Immunofluorescence Article Catalysis Cocaine and amphetamine regulated transcript Inorganic Chemistry 03 medical and health sciences parasitic diseases medicine Animals Stomach Ulcer Physical and Theoretical Chemistry Molecular Biology QD1-999 ulcer Organic Chemistry digestive system diseases 030104 developmental biology nervous system cocaine- and amphetamine-regulated transcript Enteric nervous system |
Zdroj: | International Journal of Molecular Sciences, Vol 22, Iss 7437, p 7437 (2021) International Journal of Molecular Sciences Volume 22 Issue 14 |
ISSN: | 1661-6596 1422-0067 |
Popis: | Cocaine- and amphetamine-regulated transcript (CART) is a peptide suggested to play a role in gastrointestinal tract tissue reaction to pathology. Gastric ulceration is a common disorder affecting huge number of people, and additionally, it contributes to the loss of pig livestock production. Importantly, ulceration as a focal disruption affecting deeper layers of the stomach wall differs from other gastrointestinal pathologies and should be studied individually. The pig’s gastrointestinal tract, due to its many similarities to the human counterpart, provides a valuable experimental model for studying digestive system pathologies. To date, the role of CART in gastric ulceration and the expression of the gene encoding CART in porcine gastrointestinal tube are completely unknown. Therefore, we aimed to verify the changes in the CART expression by Q-PCR (gene encoding CART in the tissue) and double immunofluorescence staining combined with confocal microscopy (CART immunofluorescence in enteric nervous system) in the porcine stomach tissues adjacent to gastric ulcerations. Surprisingly, we found that gastric ulcer caused a significant decrease in the expression of CART-encoding gene and huge reduction in the percentage of CART-immunofluorescent myenteric perikarya and neuronal fibers located within the circular muscle layer. Our results indicate a unique CART-dependent gastric response to ulcer disease. |
Databáze: | OpenAIRE |
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