| Autor: |
Xiaoxue Zhou, Enrique R. Rojas, Julie A. Theriot, David K. Halladin |
| Jazyk: |
angličtina |
| Předmět: |
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| Zdroj: |
Biophysical Journal. (2):579a |
| ISSN: |
0006-3495 |
| DOI: |
10.1016/j.bpj.2013.11.3209 |
| Popis: |
Most bacteria surround themselves with a tough cell wall made of peptidoglycan that preserves cellular integrity and maintains cell shape. Peptidoglycan must be dynamic to accommodate cell growth and division. Enzymes that hydrolyze peptidoglycan are crucial for these processes, but their activities can be lethal if not tightly controlled. In Gram-positive coccus Staphylococcus aureus, cell division can be classified into three stages: septation, daughter cell separation and finally disassociation. Previous Cryo-EM data has indicated that prior to cell separation the two daughter cells are only connected through the peripheral peptidoglycan. This result has led to the hypothesis that there are two classes of cell wall hydrolases: one class that splits the majority of the septum and the other class that resolves the final connecting ring to trigger cell separation. The identities of the hydrolases involved in these two stages and how the cell coordinates and regulates them are still not clear. We have examined the major cell wall hydrolases Atl and Sle1 in S. aureus and found that a sle1 deletion mutant is delayed in cell separation while an atl mutant separated normally but was impaired in cell disassociation.View Large Image | View Hi-Res Image | Download PowerPoint Slide |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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