Method for determination of angiotensin-converting enzyme activity in blood and tissue by high-performance liquid chromatography
| Autor: | Tatsuo Terashima, Ken-Ichi Fujimura, Masato Horiuchi, Tadashi Iso |
|---|---|
| Rok vydání: | 1982 |
| Předmět: |
Male
Guinea Pigs Peptidyl-Dipeptidase A Kidney High-performance liquid chromatography Mice chemistry.chemical_compound Dogs medicine Animals Humans Lung Chromatography High Pressure Liquid Whole blood chemistry.chemical_classification Oligopeptide Chromatography biology Chemistry Hippurates Substrate (chemistry) Hippuric acid Rats Inbred Strains General Chemistry Enzyme assay Rats medicine.anatomical_structure Enzyme Biochemistry biology.protein Rabbits Oligopeptides |
| Zdroj: | Journal of Chromatography B: Biomedical Sciences and Applications. 233:123-130 |
| ISSN: | 0378-4347 |
| DOI: | 10.1016/s0378-4347(00)81738-7 |
| Popis: | A simplified method for an angiotensin-converting enzyme activity assay in biological samples was developed. Samples were incubated with hippurylhistidylleucine, an artificial substrate of angiotensin-converting enzyme. The reaction was terminated by the addition of metaphosphoric acid and liberated hippuric acid in the supernatant was quantitated directly by reversed-phase high-performance liquid chromatography. Tissues were homogenized in the presence of Nonidet-P40, a detergent, and the resulting supernatant was used for the assay of tissue angiotensin-converting enzyme activity by high-performance liquid chromatography. The present procedure made it possible to determine angiotensin-converting enzyme activity in whole blood and the total activity in tissues. A comparative study of angiotensin-converting enzyme activity in plasma, kidney and lung of five experimental animals showed a high degree of variation from species to species. |
| Databáze: | OpenAIRE |
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