The Physico-Chemical Properties and Exploratory Real-Time Cell Analysis of Hydroxyapatite Nanopowders Substituted with Ce, Mg, Sr, and Zn (0.5–5 at.%)
| Autor: | Monica Enculescu, Ana-Maria Enciu, Adrian-Claudiu Popa, Lucian Albulescu, Cristiana Tanase, Daniela Predoi, Teddy Tite, George E. Stan, Sultana Niţă, Iuliana Maria Chirica, Cristian Radu, Iuliana Pasuk, Cătălina Gabriela Mihalcea, Simona Liliana Iconaru, Maria Dudau, Nicoleta Rusu |
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| Rok vydání: | 2021 |
| Předmět: |
Technology
Biocompatibility Composite number 02 engineering and technology 010402 general chemistry 01 natural sciences Article biocompatibility medicine ICP-MS General Materials Science structure Inductively coupled plasma mass spectrometry chemistry.chemical_classification Microscopy QC120-168.85 real-time cell analysis Chemistry QH201-278.5 substituted hydroxyapatite Osteoblast Polymer Engineering (General). Civil engineering (General) 021001 nanoscience & nanotechnology TK1-9971 0104 chemical sciences medicine.anatomical_structure Descriptive and experimental mechanics composition Transmission electron microscopy bio-functionality Composition (visual arts) Electrical engineering. Electronics. Nuclear engineering Crystallite TA1-2040 0210 nano-technology Nuclear chemistry |
| Zdroj: | Materials Materials, Vol 14, Iss 3808, p 3808 (2021) Volume 14 Issue 14 |
| ISSN: | 1996-1944 |
| Popis: | Cation-substituted hydroxyapatite (HA), standalone or as a composite (blended with polymers or metals), is currently regarded as a noteworthy candidate material for bone repair/regeneration either in the form of powders, porous scaffolds or coatings for endo-osseous dental and orthopaedic implants. As a response to the numerous contradictions reported in literature, this work presents, in one study, the physico-chemical properties and the cytocompatibility response of single cation-doped (Ce, Mg, Sr or Zn) HA nanopowders in a wide concentration range (0.5–5 at.%). The modification of composition, morphology, and structure was multiparametrically monitored via energy dispersive X-ray, X-ray photoelectron, Fourier-transform infrared and micro-Raman spectroscopy methods, as well as by transmission electron microscopy and X-ray diffraction. From a compositional point of view, Ce and Sr were well-incorporated in HA, while slight and pronounced deviations were observed for Mg and Zn, respectively. The change of the lattice parameters, crystallite size, and substituting cation occupation factors either in the Ca(I) or Ca(II) sites were further determined. Sr produced the most important HA structural changes. The in vitro biological performance was evaluated by the (i) determination of leached therapeutic cations (by inductively coupled plasma mass spectrometry) and (ii) assessment of cell behaviour by both conventional assays (e.g., proliferation—3-(4,5-dimethyl thiazol-2-yl) 5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium assay cytotoxicity—lactate dehydrogenase release assay) and, for the first time, real-time cell analysis (RTCA). Three cell lines were employed: fibroblast, osteoblast, and endothelial. When monophasic, the substituted HA supported the cells’ viability and proliferation without signs of toxicity. The RTCA results indicate the excellent adherence of cells. The study strived to offer a perspective on the behaviour of Ce-, Mg-, Sr-, or Zn-substituted HAs and to deliver a well-encompassing viewpoint on their effects. This can be highly important for the future development of such bioceramics, paving the road toward the identification of candidates with highly promising therapeutic effects. |
| Databáze: | OpenAIRE |
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