Short Report: Circulating microRNAs are associated with incident diabetes over 10 years in Japanese Americans
| Autor: | Pandora L. Wander, Edward J. Boyko, Wilfred Y. Fujimoto, Daniel A. Enquobahrie, Steven E. Kahn, Sengkeo L. Srinouanprachanh, James W. MacDonald, Theo K. Bammler, Donna L. Leonetti |
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| Rok vydání: | 2020 |
| Předmět: |
Adult
Male 0301 basic medicine Oncology medicine.medical_specialty Diabetes risk lcsh:Medicine Pilot Projects Article Pathogenesis 03 medical and health sciences 0302 clinical medicine Diabetes mellitus Internal medicine microRNA Humans Medicine Endocrine system Circulating MicroRNA Epigenetics lcsh:Science Muscle Skeletal Multidisciplinary Molecular medicine Asian business.industry lcsh:R High-Throughput Nucleotide Sequencing Middle Aged medicine.disease 030104 developmental biology Diabetes Mellitus Type 2 030220 oncology & carcinogenesis lcsh:Q Female Observational study Pre-diabetes business Biomarkers |
| Zdroj: | Scientific Reports Scientific Reports, Vol 10, Iss 1, Pp 1-6 (2020) |
| ISSN: | 2045-2322 |
| DOI: | 10.1038/s41598-020-63606-3 |
| Popis: | Epigenetic changes precede the development of diabetes by many years, providing clues to its pathogenesis. We explored whether the epigenetic markers, circulating microRNAs (miRNAs), were associated with incident diabetes in Japanese Americans. We conducted a pilot study (n = 10) using plasma from age- and sex-matched participants who did or did not develop diabetes in the Japanese American Community Diabetes Study, an observational study of diabetes risk factors. Extraction and high-throughput sequencing of miRNAs were performed using samples collected at baseline. Regression models were fit comparing circulating miRNAs (N = 1640) among individuals who did or did not develop incident diabetes at 10-year follow-up. Participants averaged 51.7 years of age at baseline; 60% were male. We identified 36 miRNAs present at different (10 higher and 26 lower) levels in individuals who developed diabetes compared to those who did not (log2fold change ≥1.25 and false discovery rate ≤5%). These included miRNAs with functions in skeletal muscle insulin metabolism (miR-106b and miR-20b-5p) and miRNAs with functions in both skeletal muscle insulin metabolism and cell cycle regulation in endocrine pancreas (miR-15a and miR-17). Circulating miRNAs were associated with subsequent development of diabetes among Japanese Americans over 10 years of follow-up. Results are preliminary. Large-scale miRNA sequencing studies could inform our understanding of diabetes pathogenesis and development of therapies, based on gene expression regulation, that target diabetes. |
| Databáze: | OpenAIRE |
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